Prokineticin 2 regulates the electrical activity of rat suprachiasmatic nuclei neurons.
Prokineticin 2 regulates the electrical activity of rat suprachiasmatic nuclei neurons.
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Prokineticin 2 调节大鼠视交叉上核神经元的电活动
DOI：
10.1371/journal.pone.0020263
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Hu WP
中科院分区：
文献类型：
--
作者：
Ren P;Zhang H;Qiu F;Liu YQ;Gu H;O'Dowd DK;Zhou QY;Hu WP
神经肽信号在协调细胞活动和维持哺乳动物视交叉上核（SCN）内的稳健振荡中起作用。Prokineticin2 （PK2）是一种来自SCN的信号分子，参与昼夜运动活动的产生。促运动素受体2 （PKR2）是PK2的受体，已被证明在SCN中表达。然而，我们对PK2在SCN内的细胞作用知之甚少。在本研究中，我们利用全细胞膜片钳记录在SCN切片上研究了PK2对自发放电和微型抑制性突触后电流（mIPSCs）的影响。PK2剂量依赖性地增加了SCN背侧大多数神经元的自发放电率。PK2突触后降低SCN内γ-氨基丁酸（GABA）能功能，PK2降低mIPSCs的振幅，但不降低其频率。此外，PK2还抑制外源性gaba诱导的电流。PK2的抑制作用需要在突触后细胞中激活PKC。我们的数据表明PK2可以改变SCN内的细胞活动，并可能影响行为和生理节律。
Neuropeptide signaling plays roles in coordinating cellular activities and maintaining robust oscillations within the mammalian suprachiasmatic nucleus (SCN). Prokineticin2 (PK2) is a signaling molecule from the SCN and involves in the generation of circadian locomotor activity. Prokineticin receptor 2 (PKR2), a receptor for PK2, has been shown to be expressed in the SCN. However, very little is known about the cellular action of PK2 within the SCN. In the present study, we investigated the effect of PK2 on spontaneous firing and miniature inhibitory postsynaptic currents (mIPSCs) using whole cell patch-clamp recording in the SCN slices. PK2 dose-dependently increased spontaneous firing rates in most neurons from the dorsal SCN. PK2 acted postsynaptically to reduce γ-aminobutyric acid (GABA)-ergic function within the SCN, and PK2 reduced the amplitude but not frequency of mIPSCs. Furthermore, PK2 also suppressed exogenous GABA-induced currents. And the inhibitory effect of PK2 required PKC activation in the postsynaptic cells. Our data suggest that PK2 could alter cellular activities within the SCN and may influence behavioral and physiological rhythms.
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影响因子：
2.5
作者：
Itri, J;Michel, S;Colwell, CS
通讯作者：
Colwell, CS
影响因子：
64.8
作者：
Cheng, MY;Bullock, CM;Zhou, QY
通讯作者：
Zhou, QY
影响因子：
3.3
作者：
Brown, T. M.;McLachlan, E.;Piggins, H. D.
通讯作者：
Piggins, H. D.
影响因子：
3.2
作者：
Inyushkin, A. N.;Bhumbra, G. S.;Dyball, R. E. J.
通讯作者：
Dyball, R. E. J.
影响因子：
16.2
作者：
KRISHEK, BJ;XIE, XM;SMART, TG
通讯作者：
SMART, TG