Lactobacillus plantarumInoculants Delay Spoilage of High Moisture Alfalfa Silages by Regulating Bacterial Community Composition

Lactobacillus plantarumInoculants Delay Spoilage of High Moisture Alfalfa Silages by Regulating Bacterial Community Composition
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植物乳杆菌接种剂通过调节细菌群落组成来延迟高水分苜蓿青贮饲料的腐败

DOI:
10.3389/fmicb.2020.01989
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发表时间:
2020-08-12
影响因子:
5.2
通讯作者:
Wang, Yuan
Wang, Yuan
中科院分区:
生物学2区
文献类型:
--
作者:
Yang, Fengyuan;Wang, Yanping;Wang, Yuan

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The objective of this study was to investigate the mechanism of Lactobacillus plantarum (L. plantarum) involved in improving fermentation quality of naturally ensiled alfalfa under poor conditions. High-moisture wilted alfalfa was ensiled without inoculants (CK) or with inoculation of two L. plantarum additives (LPI and LPII). The pH and fermentation products of silage were determined after 30 and 90 days of ensiling. Additionally, the bacterial community compositions were analyzed. The L. plantarum inoculants significantly promoted lactic acid accumulation, and Lactobacillus abundance for both periods. At 90 days, silage in CK exhibited a high pH, a loss in dry matter, and a high concentration of ammoniacal nitrogen. The inoculations of L. plantarum significantly inhibited the growth of Clostridia, and reduced ammoniacal nitrogen concentration in silage (P < 0.05). Thus, inoculation with L. plantarum improved the fermentation quality of alfalfa silage and inhibited the growth of spoilage microorganisms, and further delayed spoilage of alfalfa silage under adverse ensiling conditions.