Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models
Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models
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六种动物口腔黏膜纤维化相关分子的表达：为动物模型选择提供参考
DOI：
10.1016/j.ejps.2016.10.018
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发表时间：
2017
影响因子：
4.6
通讯作者：
He Sangang
中科院分区：
文献类型：
--
作者：
Sa Guoliang;Xiong Xuepeng;Ren Jiangang;Zhao Yifang;He Sangang
目前已有多种动物模型用于口腔黏膜下纤维性变(OSF)的相关研究，然而，基于OSF的组织病理学特征的合适的动物模型仍然是需要的。本研究旨在为选择可能的模型提供组织学参考。用免疫组织化学方法检测了I型胶原(Col I)、III型胶原(Col III)、IV型胶原(Col IV)、纤维连接蛋白(FN)、转化生长因子β(β)和结缔组织生长因子(CCN2)在人和6种非人类动物口腔黏膜中的表达强度。Col I的表达强度变化不大，而Col III、Col IV和Col Fn的表达强度有差异。转化生长因子-β在狗、大鼠、绵羊和猪的口腔粘膜中的表达强度与在人的口腔粘膜中的表达强度相当。犬口腔粘膜中纤维化相关分子的表达最能模拟人类的情况，这表明它适合作为研究OSF的组织病理学动物模型。
Several animal models have been used in studies associated with oral submucous fibrosis (OSF); however, an appropriate model based on the histopathological characteristics of OSF is still needed. This study aimed to provide histological references for selecting a potential model. The expression intensities of collagen type I (Col I), type III (Col III), type IV (Col IV), fibronectin (FN), transforming growth factors β (TGF-β), and connective tissue growth factor (CCN2) in the oral mucosa of the human and six non-human animal species were measured by immunohistochemistry. There was little variation in the expression intensity of Col I while the expression of Col III, Col IV, and FN showed differences. The expression intensities of TGF-β in dog, rat, sheep, and pig oral mucosae, and those of CCN2 in dog, minipig, rat, and buffalo oral mucosae were equivalent to the expression intensities in human mucosa. The expression of fibrosis-related molecules in the dog oral mucosa optimally mimics the human condition, suggesting its suitability with regard to histopathology as an animal model for the study of OSF.