Induction of single cell stage in tissue cultured shoot primordia of Haplopappus gracilis (2n=4), a preliminary reports1)

Induction of single cell stage in tissue cultured shoot primordia of Haplopappus gracilis (2n=4), a preliminary reports1)
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Haplopappus gracilis组织培养芽原基的单细胞阶段诱导(2n=4),初步报告1)

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发表时间:
1985
期刊:
影响因子:
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通讯作者:
Y. Kamisugi
Y. Kamisugi
中科院分区:
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文献类型:
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作者:
R. Tanaka;K. Taniguchi;Y. Kamisugi

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从组织培养的细单冠状茎原基中诱导出游离和悬浮状态的单细胞(2n=4)。单细胞悬浮液在Tanaka和Ikeda(1983)报道的原茎原基培养条件下培养,可形成新的茎原基。提出了植物组织培养体细胞生活史微繁殖的新途径,即茎原基→单细胞悬浮液→再生茎原基→小植株→茎原基。
Single cells in a free and suspended state were induced from the tissue cultured shoot primordia of Haplopappus gracilis (2n=4). New shoot primordia were formed from the suspensions of single cells, when the suspensions were cultured in the conditions of original shoot primordia reported by Tanaka and Ikeda (1983). A new path of micropropagation in somatic life cycle in plant tissue culture was proposed, that is shoot primordia→suspensions of single cells→regenerated shoot primordia→plantlets→shoot primordia.