ANALYSIS OF INDUCED SPUTUM TO EXAMINE THE EFFECTS OF PREDNISONE ON AIRWAY INFLAMMATION IN ASTHMATIC SUBJECTS
ANALYSIS OF INDUCED SPUTUM TO EXAMINE THE EFFECTS OF PREDNISONE ON AIRWAY INFLAMMATION IN ASTHMATIC SUBJECTS
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DOI：
10.1016/0091-6749(94)90154-6
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发表时间：
1994-11-01
影响因子：
14.2
通讯作者：
FAHY, JV
中科院分区：
文献类型：
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作者：
CLAMAN, DM;BOUSHEY, HA;FAHY, JV
为了确定诱导痰样本是否可以提供一个有用的方法来评估治疗对气道粘膜炎症的影响，我们在一项随机、双盲研究中，对24名哮喘受试者进行了泼尼松（0.5 mg/kg/d）或安慰剂治疗6天前后的诱导痰样本进行了检查。分析诱导痰的细胞总数和分类计数以及嗜酸性粒细胞阳离子蛋白、白蛋白和粘蛋白样糖蛋白的浓度。我们发现，泼尼松治疗组痰液样本中嗜酸性粒细胞的平均（+/- SEM）百分比从基线时的14.1% +/- 5.0%下降到治疗后的1.8% +/- 0.8%，降幅显著大于安慰剂治疗组（从10.3% +/- 4.9%至11.1% +/- 4.0%; p = 0.002）。与安慰剂治疗组相比，泼尼松治疗组中嗜酸性粒细胞的绝对数量也显著减少（p = 0.04）。此外，泼尼松治疗组诱导痰中嗜酸性粒细胞阳离子蛋白水平比安慰剂治疗组下降更多（从324 +/- 131 ng/ml至144 +/- 84 ng/ml vs 173 +/- 50 ng/ml至188 +/- 47 ng/ml; p = 0.002）。此外，泼尼松治疗与呼气峰流量的显着增加有关，其效果与呼吸道阻力的降低显着相关。诱导痰中嗜酸性粒细胞百分比（r（s）= 0.64，p = 0.04）。泼尼松治疗与白蛋白或粘蛋白样糖蛋白浓度的任何显著变化无关。我们的结论是，分析诱导痰是一个有用的非侵入性的方法来研究哮喘治疗对气道嗜酸性粒细胞炎症的影响。
To detemine whether induced sputum samples might provide a useful means for evaluating the effects of therapy on airway mucosal inflammation, we examined induced sputum samples obtained before and after 6 days of treatment with prednisone (0.5 mg/kg/day) or placebo in a randomized, double-blind study of 24 asthmatic subjects. Induced sputum was analyzed for total and differential cell counts and for concentrations of eosinophil cationic protein, albumin, and mucin-like glycoprotein. We found that the mean (+/- SEM) percentage of eosinophils in sputum samples from the prednisone-treated group fell from 14.1% +/- 5.0% at baseline to 1.8% +/- 0.8% after treatment, a decrease significantly greater than in the placebo-treated group (from 10.3% +/- 4.9% to 11.1% +/- 4.0%; p = 0.002). The absolute number of eosinophils also decreased significantly more in the prednisone-treated group than in the placebo-treated group (p = 0.04). In addition, eosinophil cationic protein levels in induced sputum fell more in the prednisone-treated group than in the placebo-treated group (from 324 +/- 131 ng/ml to 144 +/- 84 ng/ml vs 173 +/- 50 ng/ml to 188 +/- 47 ng/ml; p = 0.002). Furthermore prednisone treatment was associated with a significant increase in peak expiratory flow an effect chat was significantly correlated with the decrease in. eosinophil percentage in induced sputum (r(s) = 0.64, p = 0.04). Prednisone treatment was not associated with any significant change in the concentrations of albumin or mucin-like glycoprotein. We conclude that analysis of induced sputum is a useful noninvasive method for studying the effects of asthma therapy on airway eosinophilic inflammation.