Delayed dissemination of Lyme disease spirochetes from the site of deposition in the skin of mice.
Delayed dissemination of Lyme disease spirochetes from the site of deposition in the skin of mice.
复制标题
莱姆病螺旋体从小鼠皮肤沉积部位的延迟传播。
DOI：
10.1093/infdis/166.4.827
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Spielman,A
中科院分区：
文献类型：
--
作者：
Shih,CM;Pollack,RJ;Telford3rd,SR;Spielman,A
为了确定莱姆病病原体是否直接在脊椎动物宿主中传播，在感染莱姆病螺旋体伯氏疏螺旋体后，从感染莱姆病螺旋体的若虫感染部位切下一张6毫米长的皮肤圆盘。通过检查4周后在这些小鼠身上充血的异种诊断扁虱和血清学测试来测试每只小鼠的感染情况。若接种部位在分离后2天内切除，则全身感染即告流产，但2周后未见异常。相比之下，当咬伤部位保持完好时，所有小鼠都会受到感染。只有当样本在感染扁虱后一周内被消融时，才能从附着部位周围的组织中培养出螺旋体。这些观察表明，感染扁虱将莱姆病的病原体直接传递到皮肤中，这种螺旋体在传播到偏远地区之前在当地繁殖了几天。
To determine whether the agent of Lyme disease disseminates in vertebrate hosts directly after deposition by an infecting tick, a 6-mm disk of skin was excised from the sites where nymphalIxodes damminiticks infected by Lyme disease spirochetes,Borrelia burgdorferi, had fed. Infection in each mouse was tested by examining xenodiagnostic ticks that had engorged on these mice 4 weeks later and by serologic testing. Generalized infection was aborted when the site of inoculation was excised within 2 days after the infecting tick detached but not after 2 weeks. In contrast, all mice became infected when the bite site remained intact. Spirochetes could be cultured from the tissues around the site of attachment solely when the sample was ablated within a week after infecting ticks detached. These observations suggest that infecting ticks deliver the agent of Lyme disease directly into the skin and that such spirochetes multiply locally for some days before disseminating to remote sites.