PHYSICOCHEMICAL AND MORPHOLOGICAL RELATIONSHIPS OF SOME ARTHROPOD-BORNE IRUSES TO BLUETONGUE VIRUS - NEW TAXONOMIC GROUP - PHYSICOCHEMICAL AND SEROLOGICAL STUDIES
PHYSICOCHEMICAL AND MORPHOLOGICAL RELATIONSHIPS OF SOME ARTHROPOD-BORNE IRUSES TO BLUETONGUE VIRUS - NEW TAXONOMIC GROUP - PHYSICOCHEMICAL AND SEROLOGICAL STUDIES
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DOI：
10.1099/0022-1317-13-2-261
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发表时间：
1971-01-01
影响因子：
3.8
通讯作者：
MURPHY, FA
中科院分区：
文献类型：
--
作者：
BORDEN, EC;SHOPE, RE;MURPHY, FA
根据对脂质溶剂和脱氧胆酸钠的相对稳定性、在pH 3.0下的不稳定性以及与主要虫媒病毒血清组A、B和Bunyamwera缺乏抗原关系，对几种节肢动物传播的病毒进行了分组。这些病毒以前未分组或在次要血清群中，包括蓝舌病、鹿流行性出血病、Eubenangee、IbAr 22619、B1327、科罗拉多蜱热、非洲马瘟、Irituia、Changuinola、BeAr 35646、BeAr 41067、凯梅罗沃、Chenuda、Tribec、Wad Medani、Mono Lake、Huacho、Lebombo、Palyam、达吉拉尔、G8886、G15534、Corriparta、Acado、MP359、CH9935和MRM 10434。与其他虫媒病毒相比，脂质溶剂或脱氧胆酸钠引起的这些病毒的感染性降低小于101.5乳鼠脑内LD 50。这种程度的敏感性是可重复的，是显着不同的呼肠孤病毒和小核糖核酸病毒的绝对电阻。将这些病毒中的每一种暴露于pH 3.0 3小时后，未恢复残余感染性。通过补体结合试验，没有发现与其他病毒分类群成员的血清学关系，这些病毒本身没有共同的群抗原，但根据个体交叉反应被分为10个血清学上不同的亚群。虽然可能含有双链RNA基因组，但相对耐溶剂的虫媒病毒与呼肠孤病毒的区别在于酸不稳定性、轻微的溶剂敏感性和血清学。
Several arthropod-borne viruses were grouped on the basis of relative stability to lipid solvents and sodium deoxycholate, lability at pH 3.0, and lack of antigenic relationship to major arbovirus serologic groups A, B, and Bunyamwera. These viruses, previously ungrouped or in minor serogroups, include bluetongue, epizootic haemorrhagic disease of deer, Eubenangee, IbAr 22619, B1327, Colorado tick fever, African horse sickness, Irituia, Changuinola, BeAr 35646, BeAr 41067, Kemerovo, Chenuda, Tribec, Wad Medani, Mono Lake, Huacho, Lebombo, Palyam, D’Aguilar, G8886, G15534, Corriparta, Acado, MP359, CH9935, and MRM 10434. In contrast to other arboviruses, the reduction in infectivity of these viruses caused by lipid solvents or sodium deoxycholate was less than 101.5suckling mouse intracerebral LD50. This degree of sensitivity was reproducible and was significantly different from the absolute resistance of reoviruses and picorna-viruses. After exposure of each of these viruses to pH 3.0 for 3 hr, no residual infectivity was recovered. By complement-fixation testing no serological relationship to members of other virus taxonomic groups was found. The viruses themselves had no common group antigen but were placed into ten serologically distinct subgroups on the basis of individual cross-reactions. Although probably containing a double-stranded RNA genome, the relatively solvent resistant arboviruses were distinguished from reoviruses by acid lability, slight solvent sensitivity, and serology.