In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus
In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus
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Griffithsin对猪流行性腹泻病毒的体外抗病毒活性
DOI：
10.1007/s11262-019-01633-7
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发表时间：
2019-04-01
期刊：
影响因子：
1.6
通讯作者：
Hou, Jibo
中科院分区：
文献类型：
--
作者：
Li, Lan;Yu, Xiaoming;Hou, Jibo
Griffithsin是一种对包膜病毒具有有效抗病毒活性的凝集素。本研究的目的是评价Griffithsin对猪流行性腹泻病毒（PEDV）的抑制作用。结果表明，Griffithsin可使Vero细胞的PEDV感染降低约82.8%。此外，通过添加时间测定和RT-qPCR，我们发现延迟添加Griffithsin对PEDV的抑制作用比早期治疗弱。Griffithsin抗PEDV的作用机制包括阻止病毒附着到宿主细胞和破坏细胞间传播；它的双重作用模式使Griffithsin区别于大多数其他抗病毒药物。综上所述，Griffithsin是一种有效的PEDV抑制剂，可能是预防PEDV感染的候选药物。
Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin's inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin's action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.