Adapting the algal microbiome for growth on domestic landfill leachate.
Adapting the algal microbiome for growth on domestic landfill leachate.
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DOI：
10.1016/j.biortech.2020.124246
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发表时间：
2021-01
影响因子：
11.4
通讯作者：
K. Okurowska;E. Karunakaran;A. AL-fartoosy;Narciso Couto;J. Pandhal
中科院分区：
文献类型：
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作者：
K. Okurowska;E. Karunakaran;A. AL-fartoosy;Narciso Couto;J. Pandhal
我们的目标是通过优化藻类微生物组来提高渗滤液中的藻类生长率。从垃圾填埋场渗滤液中富集了藻类-细菌群落，并经过 24 个月的适应性实验室进化，将优势藻类普通小球藻的生长速度提高了几乎三倍，达到 0.2 d−1。硝酸盐产量的急剧减少表明氨氮的生物利用发生了转变，这得到了分子 16S rRNA 分类学分析的支持，其中在适应的群落中没有检测到亚硝化单胞菌数量。 PICRUSt 方法预测了宏基因组功能内容，并揭示了属于生物修复途径的大量序列，包括芳香族化合物、苯甲酸盐和萘的降解，以及已知参与藻类-细菌共生的途径。这项研究增强了我们对复杂污水中藻类-细菌关联的有益机制的理解，并最终实现了优化藻类微生物组的自下而上设计，以供工业内开发。
We aimed to improve algal growth rate on leachate by optimising the algal microbiome. An algal-bacterial consortium was enriched from landfill leachate and subjected to 24 months of adaptive laboratory evolution, increasing the growth rate of the dominant algal strain,Chlorella vulgaris, almost three-fold to 0.2 d−1. A dramatic reduction in nitrate production suggested a shift in biological utilisation of ammoniacal-N, supported by molecular 16S rRNA taxonomic analyses, where Nitrosomonas numbers were not detected in the adapted consortium. A PICRUSt approach predicted metagenomic functional content and revealed a high number of sequences belonging to bioremediation pathways, including degradation of aromatic compounds, benzoate and naphthalene, as well as pathways known to be involved in algal-bacterial symbiosis. This study enhances our understanding of beneficial mechanisms in algal-bacterial associations in complex effluents, and ultimately enables the bottom-up design of optimised algal microbiomes for exploitation within industry.