NEW SPECIES OF VIRUS-CODED LOW-MOLECULAR WEIGHT RNA FROM CELLS INFECTED WITH ADENOVIRUS TYPE-2
NEW SPECIES OF VIRUS-CODED LOW-MOLECULAR WEIGHT RNA FROM CELLS INFECTED WITH ADENOVIRUS TYPE-2
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DOI：
10.1016/0092-8674(76)90209-9
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发表时间：
1976-01-01
期刊：
影响因子：
64.5
通讯作者：
MATHEWS, MB
中科院分区：
文献类型：
--
作者：
SODERLUND, H;PETTERSSON, U;MATHEWS, MB
从感染了2型腺病毒的海拉细胞中分离出一种病毒编码的低分子量RNA（5.2S），它在聚丙烯酰胺凝胶上的迁移速度比特征明确的腺病毒特异性5.5S RNA略快。杂交竞争实验和RNA指纹图谱表明，这两种病毒相关（VA）RNA的一级结构不同。5.2S RNA的基因位于5.5S RNA基因的右侧，在2型腺病毒DNA图谱上位于30.3和32.2位置之间的一段DNA的l链上。在感染后早期以及在存在阿糖胞苷或环己酰亚胺的情况下都能检测到5.5S和5.2S RNA。在病毒DNA复制开始后，5.2S RNA的合成趋于平稳，而5.5S RNA的合成量则不断增加。5.2S和5.5S RNA都是在分离的细胞核中由一种对α -鹅膏蕈碱的敏感性类似于RNA聚合酶III的酶合成的。在分离的细胞核中，这两种RNA都能用β - ³²P标记的GTP进行标记，这表明它们是在不同的启动子位点起始的。
A virus-coded low MW RNA (5.2S), which migrates slightly faster on polyacrylamide gels than the well characterized adenovirus-specific 5.5S RNA, was isolated from HeLa cells infected with adenovirus type 2. Hybridization-competition experiments and RNA fingerprints indicate that the 2 virus-associated (VA) RNAs differ in their primary structures. The gene for 5.2S RNA is located to the right of the gene for 5.5S RNA, on the l strand of a DNA segment which extends between positions 30.3 and 32.2 on the map of adenovirus type 2 DNA. Both 5.5S and 5.2S RNA can be detected early after infection and also in the presence of cytosine-arabinoside or cycloheximide. After the onset of viral DNA replication, the synthesis of 5.2S RNA levels off, whereas 5.5S RNA is synthesized in increasing amounts. Both 5.2S and 5.5S RNAs are synthesized in isolated nuclei by an enzyme which resembles RNA polymerase III in its sensitivity to .alpha.-amanitin. In isolated nuclei, both RNA species are labeled with .beta.-32P-labeled GTP, which suggests that they are initiated at separate promoter sites.