NEURAMINIDASE‐RELEASABLE SURFACE SIALIC ACID OF CULTURED ASTROBLASTS EXPOSED TO ETHANOL
NEURAMINIDASE‐RELEASABLE SURFACE SIALIC ACID OF CULTURED ASTROBLASTS EXPOSED TO ETHANOL
复制标题
暴露于乙醇的培养星形细胞的神经氨酸酶可释放表面唾液酸
DOI：
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发表时间：
1976
影响因子：
4.7
通讯作者：
A. Rosenberg
中科院分区：
文献类型：
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作者：
E. P. Noble;P. Syapin;R. Vigran;A. Rosenberg
翻译后摘要-慢性暴露的完整培养的原代仓鼠成星细胞，克隆线NN，100 mM乙醇导致显着增加（10-52%）的唾液酸从细胞的释放外源性添加产气荚膜梭菌神经氨酸酶。酶促释放的唾液酸是从糖蛋白，但不是神经节苷脂部分的细胞。向培养基中添加乙醇不影响唾液酸乳糖的酶促水解，在0-200 mM的浓度范围内，乙醇也不改变唾液酸从完整NN细胞的酶促释放。总细胞唾液酸含量是独立的细胞暴露于乙醇的长度，但随着细胞的年龄而变化。这些数据表明，细胞长期暴露于乙醇导致表面糖蛋白的空间修饰。
Abstract— Chronic exposure of intact cultured primary hamster astroblasts, clonal line NN, to 100 mM ethanol resulted in significant increases (10–52%) in the releasability of sialic acid from the cell by exogenously added Clostridium perfringens neuraminidase. Enzymatically releasable sialic acid was from the glycoprotein but not the ganglioside fraction of the cell. Addition of ethanol to the medium did not affect the enzymatic hydrolysis of sialyllactose nor did ethanol, over a concentration range of 0–200 mM, alter the enzymatic release of sialic acid from intact NN cells. The total cellular sialic acid content was independent of length of cell exposure to ethanol but varied with the age of the cells. These data suggest that exposure of cells for prolonged periods to ethanol results in steric modification of surface glycoproteins.