The phytochrome A specific signaling component PAT3 is a positive regulator of Arabidopsis photomorphogenesis.
The phytochrome A specific signaling component PAT3 is a positive regulator of Arabidopsis photomorphogenesis.
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光敏色素 A 特异性信号成分 PAT3 是拟南芥光形态发生的正调节因子。
DOI：
10.1093/pcp/pce177
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发表时间：
2001
影响因子：
4.9
通讯作者：
Chua,NH
中科院分区：
文献类型：
--
作者：
Zeidler,M;Bolle,C;Chua,NH
光敏色素A通过触发从黄化生长到绿化的过渡在早期幼苗发育中起主要作用。在恒定远红光（FR）下萌发的幼苗表现出部分去黄化的表型，这在phyAmutaments中是看不到的。该光敏色素A特异性反应被用于筛选T-DNA诱变的拟南芥幼苗群体。一个突变株系pat 3（光敏色素A信号转导3）在FR光条件下不表现出下胚轴伸长的抑制，也不表现出FR诱导的杀伤反应，其在609 bp的ORF中含有T-DNA插入。该隐性突变与T-DNA抗性标记共分离，可能与tofhy 1等位。PAT 3基因组中一个2,248 bp的片段可以与突变体的表型互补，PAT 3基因在种子萌发后3 d达到最高表达，光照可使其表达下调。PAT 3与任何已知的蛋白质没有显著的同源性，并且没有显示出优先的细胞定位。当与GAL 4 DNA结合结构域融合时，该蛋白可以激活酵母中的转录。我们的研究结果表明，PAT 3是光敏色素A信号转导的正调控因子。
Phytochrome A plays a major role in early seedling development by triggering the transition from etiolated growth to greening. Seedlings germinated under constant far-red (FR) light show a partially de-etiolated phenotype that is not seen inphyAmutants. This phytochrome A specific response was used to screen a population of T-DNA mutagenized Arabidopsis seedlings. One mutant line,pat3(phytochrome A signal transduction3), which showed no inhibition of hypocotyl elongation under FR light conditions and no FR-induced killing response, contained a T-DNA insertion in a 609-bp ORF. The recessive mutation co-segregated with the T-DNA resistance marker and could be allelic tofhy1. A 2,248-bp genomic fragment of thePAT3locus can complement thepat3mutant phenotype.PAT3transcript peaked 3 d after germination and was downregulated by light. PAT3 has no significant homology to any known protein and shows no preferential cellular localization. The protein can activate transcription in yeast when fused to the GAL4 DNA-binding domain. Our results show that PAT3 is a positive regulator of phytochrome A signal transduction.