Fifteen years of Belgian experience with external quality assessment of semen analysis
Fifteen years of Belgian experience with external quality assessment of semen analysis
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DOI：
10.1111/andr.12230
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发表时间：
2016-11-01
期刊：
影响因子：
4.5
通讯作者：
Verheyen, G.
中科院分区：
文献类型：
--
作者：
Punjabi, U.;Wyns, C.;Verheyen, G.
精液分析很难标准化，质量控制和质量保证是必要的，以确保结果准确和精确。这项比利时EQA调查历时15年，涉及121个实验室，试图减少实验室间的变异性，同时鼓励参与的实验室按照世卫组织的建议采用正确的技术。在整个研究期间，精子计数的中位变异系数（CV）为19.2%，与使用的方法无关，而使用改进的Neubauer室导致中位CV显著（p < 0.001）降低（14.4%）。快速前向运动的总体中位CV较高（37.1%），但前向运动（15.1%）和总运动（13.8%）可接受。精子形态显示79.4%的变异性很大，与染色程序或使用的评价标准无关。参加比利时环境质量评估是自愿的。为了认证和患者治疗的利益，参与和实施正确的技术应是强制性的。比利时现有的EQA计划现在应该与欧洲其他现有的EQA计划协调一致。
Semen analysis is difficult to standardize, quality control and quality assurance are necessary to ensure that results are accurate and precise. This Belgian EQA survey over a 15-year period, involving 121 laboratories, attempted to reduce interlaboratory variability and at the same time, encouraged participating laboratories to implement correct techniques as advised by the WHO. Over the total period, the median coefficient of variation (CV) for sperm count, irrespective of the method used was 19.2%, while using improved Neubauer chamber resulted in a significantly (p < 0.001) lower median CV (14.4%). The overall median CV for rapid progressive motility was high (37.1%), but progressive motility (15.1%) and total motility (13.8%) were acceptable. Sperm morphology revealed a large variability in 79.4% irrespective of the staining procedures or evaluation criteria used. Participation in the Belgian EQA is on voluntary basis. Both, participation and implementation of the correct techniques should be made mandatory for accreditation and benefit of patient treatment. The existing Belgian EQA program should now be harmonized with other existing EQA schemes in Europe.