Targeting BCRP/ABCG2 by RNA interference enhances the chemotherapy sensitivity of human colon cancer side population cells
Targeting BCRP/ABCG2 by RNA interference enhances the chemotherapy sensitivity of human colon cancer side population cells
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DOI：
10.1007/s11596-017-1720-1
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发表时间：
2017-04-01
影响因子：
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通讯作者：
Kong, Da-lu
中科院分区：
文献类型：
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作者：
Hu, Jun;Li, Jian;Kong, Da-lu
结肠癌复发和转移很常见，可能与干细胞特征有关。本研究从结肠癌细胞系 (Colo-320) 中分离出侧群 (SP) 细胞，并检查其自我更新和分化能力。与非SP（NSP）细胞相比，SP结肠癌细胞在体内更具致瘤性，表现出更强的侵袭性特征和更强的形成集落的能力。此外，更多的细胞处于G(0)/G(1)期，并且更高水平地表达多药耐药蛋白BCRP/ABCG2。我们通过用靶向 BCRP/ABCG2 的发夹状小干扰 RNA (siRNA) 真核表达质粒转染 SP 细胞，实现了化疗敏感性的增强。
Relapse and metastasis are frequent in colon cancer and may be linked to stem cell characteristics. This study isolated side population (SP) cells from a colon cancer cell line (Colo-320) and examined their self-renewal and differentiation abilities. Compared to non-SP (NSP) cells, SP colon cancer cells were more tumorigenic in vivo and exhibited more invasive characteristics and a greater ability to form colonies. Additionally, more cells were in G(0)/G(1) phase and more highly expressed the multidrug resistance protein BCRP/ABCG2. We achieved enhanced chemotherapy sensitivity by transfecting SP cells with a hairpin-like, small interfering RNA (siRNA) eukaryotic expression plasmid targeting BCRP/ABCG2.