Recombinant inbred strain and interspecific backcross analysis of molecular markers flanking the murine agouti coat color locus.
Recombinant inbred strain and interspecific backcross analysis of molecular markers flanking the murine agouti coat color locus.
复制标题
鼠刺豚鼠毛色位点侧翼分子标记的重组近交系和种间回交分析。
DOI：
10.1093/genetics/122.3.669
复制
发表时间：
1989
期刊：
影响因子：
3.3
通讯作者：
Jenkins,NA
中科院分区：
文献类型：
--
作者：
Siracusa,LD;Buchberg,AM;Copeland,NG;Jenkins,NA
利用重组近交系和种间回交小鼠构建了小鼠2号染色体远端部分的分子遗传连锁图。确定Ada、Emv-13、Emv-15、Hck-1、Il-1a、Pck-1、Psp、Src-1和Svp-1基因座与β 2-微球蛋白基因座和agglutinin基因座的方向和距离。我们的映射结果提供了近端和远端的agglutinase位点的分子标记的识别。获得的重组体提供了宝贵的资源，用于确定染色体步移实验的方向，设计克隆序列的agglutinase基因座。小鼠和人类基因组图谱之间的比较表明，人类同源的agglutinase基因座位于人类染色体20 q上。还鉴定了小鼠2号染色体上不存在的三个基因座，并暂时命名为Psp-2、Hck-2和Hck-3。Psp-2基因座定位于小鼠14号染色体。Hck-2基因座定位于小鼠4号染色体的着丝粒附近，可识别林恩基因座。Hck-3基因座位于小鼠4号染色体远端附近，可识别Lck基因座。
Recombinant inbred strain and interspecific backcross mice were used to create a molecular genetic linkage map of the distal portion of mouse chromosome 2. The orientation and distance of the Ada, Emv-13, Emv-15, Hck-1, Il-1a, Pck-1, Psp, Src-1 and Svp-1 loci from the beta 2-microglobulin locus and the agouti locus were established. Our mapping results have provided the identification of molecular markers both proximal and distal to the agouti locus. The recombinants obtained provide valuable resources for determining the direction of chromosome walking experiments designed to clone sequences at the agouti locus. Comparisons between the mouse and human genome maps suggest that the human homolog of the agouti locus resides on human chromosome 20q. Three loci not present on mouse chromosome 2 were also identified and were provisionally named Psp-2, Hck-2 and Hck-3. The Psp-2 locus maps to mouse chromosome 14. The Hck-2 locus maps near the centromere of mouse chromosome 4 and may identify the Lyn locus. The Hck-3 locus maps near the distal end of mouse chromosome 4 and may identify the Lck locus.