Insecticide resistance in the Anopheles gambiae complex in Benin: a nationwide survey
Insecticide resistance in the Anopheles gambiae complex in Benin: a nationwide survey
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DOI：
10.1111/j.1365-2915.2010.00925.x
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发表时间：
2011-09-01
影响因子：
1.9
通讯作者：
Chandre, F.
中科院分区：
文献类型：
--
作者：
Djogbenou, L.;Pasteur, N.;Chandre, F.
贝宁已接受世界卫生组织建议的控制疟疾的预防策略。其国家疟疾控制计划正在实施和/或协调各种行动，并对蚊子控制策略进行评估试验。蚊虫控制基于使用经杀虫剂处理的蚊帐和室内滞留喷洒，但这些策略控制疟疾病媒的功效因杀虫剂耐药性而受到威胁。在这里，我们介绍了一项关于冈比亚按蚊杀虫剂敏感性和耐药性状况的全国性调查结果。 （双翅目：蚊科）于 2006-2007 年在贝宁进行（即在进行广泛的病媒控制之前）。总体而言，我们的研究表明，S 分子形式的蚊。冈比亚 s. s。 M 型在全国范围内广泛分布，但随着纬度的增加，M 型的出现频率明显下降。评估了对滴滴涕、氯菊酯、丁硫克百威和甲基毒死蜱的敏感性；对个体蚊子进行了物种和分子形式鉴定，并对 kdr 和 ace-1 基因座进行了基因分型。记录了对甲基毒死蜱的完全敏感性，并且极少数样品表现出对克百威的抗性。大多数样品都检测到对氯菊酯的高耐药性，并且几乎所有样品都表现出对滴滴涕的耐药性。 kdr-Leu-Phe 突变存在于 An 的所有部位和两种分子形式中。冈比亚 s. s。此外，ace-1(R)突变在S型中占主导地位，但在M型中不存在。相比之下，在阿拉伯按蚊中没有观察到目标修饰。 An 中的阻力。本研究中的冈比亚 S 分子形式似乎与农业实践有关。我们的研究表明了重要的地理差异，在贝宁不同地区应用的病媒控制策略中必须考虑到这一差异。它还强调需要定期监测全国范围内的杀虫剂耐药性并采取措施来管理耐药性。
Benin has embraced World Health Organization-recommended preventive strategies to control malaria. Its National Malaria Control Programme is implementing and/or coordinating various actions and conducting evaluation trials of mosquito control strategies. Mosquito control is based on the use of insecticide-treated nets and indoor residual spraying, but the efficacy of these strategies to control malaria vectors is endangered by insecticide resistance. Here, we present the results of a nationwide survey on the status of insecticide susceptibility and resistance in Anopheles gambiae s.l. (Diptera: Culicidae) carried out in Benin in 2006-2007 (i.e. before extensive vector control was undertaken).Overall, our study showed that the S molecular form of An. gambiae s. s. predominates and is widely distributed across the country, whereas the frequency of the M form shows a strong decline with increasing latitude. Susceptibility to DDT, permethrin, carbosulfan and chlorpyrifos-methyl was assessed; individual mosquitoes were identified for species and molecular forms, and genotyped for the kdr and ace-1 loci. Full susceptibility to chlorpyrifos-methyl was recorded and very few samples displayed resistance to carbosulfan. High resistance levels to permethrin were detected in most samples and almost all samples displayed resistance to DDT. The kdr-Leu-Phe mutation was present in all localities and in both molecular forms of An. gambiae s. s. Furthermore, the ace-1(R) mutation was predominant in the S form, but absent from the M form. By contrast, no target modification was observed in Anopheles arabiensis. Resistance in the An. gambiae S molecular form in this study seemed to be associated with agricultural practices.Our study showed important geographic variations which must be taken into account in the vector control strategies that will be applied in different regions of Benin. It also emphasizes the need to regularly monitor insecticide resistance across the country and to adapt measures to manage resistance.