Monoclonal antibodies to titin in conjunction with antibodies to desmin separate rhabdomyosarcomas from other tumor types.
Monoclonal antibodies to titin in conjunction with antibodies to desmin separate rhabdomyosarcomas from other tumor types.
复制标题
肌联蛋白单克隆抗体与结蛋白抗体结合，可将横纹肌肉瘤与其他肿瘤类型分开。
DOI：
--
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
K. Weber
中科院分区：
文献类型：
--
作者：
M. Osborn;C. Hill;M. Altmannsberger;K. Weber
肌联蛋白是肌节肌肉的主要组成蛋白，并且被认为产生肌原纤维组织下面的弹性丝组分。肌联蛋白的单克隆抗体已在正常和病理性人体材料以及培养的人细胞系上进行了表征。阳性免疫细胞化学反应仅限于肌节，内脏或血管平滑肌或各种非肌肉组织上不发生。当不同的肿瘤类型进行了检查肌联蛋白抗体只与横纹肌肉瘤反应，并没有与平滑肌肉瘤或平滑肌瘤，或与非肌肉肿瘤类型的测试。在横纹肌肉瘤中，肌联蛋白抗体阳性细胞明显少于结蛋白抗体阳性细胞，表明横纹肌肉瘤中的单个细胞实现了不同程度的肌源性分化。结果加强了使用结蛋白作为肌肉肉瘤的标志物，并表明，横纹肌肉瘤的阳性鉴定可以通过免疫细胞化学与平行使用结蛋白和肌联蛋白抗体。
Titin is a major constituent protein of sarcomeric muscles and is thought to give rise to an elastic filament component underlying the myofibrillar organization. Monoclonal antibodies to titin have been characterized on normal and pathological human material and on human cell lines in culture. A positive immunocytochemical reaction was restricted to sarcomeric muscles and did not occur on visceral or vascular smooth muscles or on various nonmuscle tissues. When different tumor types were examined titin antibodies reacted solely with rhabdomyosarcomas and did not react with leiomyosarcoma or leiomyoma, or with the nonmuscle tumor types tested. In rhabdomyosarcomas a noticeably smaller population of cells were positive with antibodies to titin than with antibodies to desmin showing that individual cells within a rhabdomyosarcoma achieve different degrees of myogenic differentiation. The results reinforce the use of desmin as a marker for muscle sarcomas and show that a positive identification of rhabdomyosarcoma can be achieved by immunocytochemistry with the parallel use of desmin and titin antibodies.