IDIOTOPE-SPECIFIC T-CELL CLONES THAT RECOGNIZE SYNGENEIC IMMUNOGLOBULIN FRAGMENTS IN THE CONTEXT OF CLASS-II MOLECULES
IDIOTOPE-SPECIFIC T-CELL CLONES THAT RECOGNIZE SYNGENEIC IMMUNOGLOBULIN FRAGMENTS IN THE CONTEXT OF CLASS-II MOLECULES
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DOI：
10.1002/eji.1830161110
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发表时间：
1986-11-01
影响因子：
5.4
通讯作者：
HAAS, W
中科院分区：
文献类型：
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作者：
BOGEN, B;MALISSEN, B;HAAS, W
我们已经建立了独特位（Id）特异性T细胞系和克隆，它们来源于用BALB/c骨髓瘤蛋白M315（α，β）的轻链（λ 2315）免疫的至少4种不同BALB/c小鼠。（见附件2）。独立衍生的克隆是不可区分的，因为它们与V.2315反应，对应于第三高变区的体细胞突变的密码子94、95和96的一个或多个氨基酸对于Id的表达是必需的。虽然Id在V.2315 Fv 315和V.2315片段上有效表达，需要高约100-1000倍摩尔浓度的Fab 315和M315来诱导等效的应答。因此，IG四级结构严重影响T细胞Id的可用性。V.E.2315特异性T细胞是Thy-1.2+、L3 T4+、Ly-2.2-和I-Ed限制性的。一些T细胞克隆在活化后产生白细胞介素2（IL 2）、IL 3和B细胞生长和分化因子。此外，T细胞在长期试验中具有细胞毒性。图形 **。但不是。**图形 **。细胞毒性作用是T细胞活化的L细胞生长抑制测定的基础，其比普通增殖测定灵敏至少10倍。
We have established idiotope (Id)-specific T cell lines and clones derived from at least 4 different BALB/c mice immunized with the light chain (.lambda.2315) of the BALB/c myeloma protein M315 (.alpha.,.lambda.2). Independently derived clones were indistinguishable in that they reacted to V.lambda.2315, one or more of the amino acids corresponding to somatically mutated codons 94, 95 and 96 of the third hypervariable region being essential for expression of the Id. While the Id was efficiently expressed on V.lambda.2315 Fv315 and .lambda.2315 fragments, about a 100-1000-fold higher molar concentration of Fab315 and M315 was needed to induce equivalent responses. Thus, Ig quaternary structure heavily influenced the availability of the Id for T cells. The V.lambda.2315-specific T cells were Thy-1.2+, L3T4+, Ly-2.2- and I-Ed restricted. Some of the T cell clones produced interleukin 2 (IL2), IL3 and B cell growth and differentiation factors upon activation. In addition, T cells were cytotoxic in long-term assays for .**GRAPHIC**. but not .**GRAPHIC**. transfected L cells in the presence of Id. The cytotoxic effect was the basis for an L cell growth inhibition assay for T cell activation that was at least 10-fold more sensitive than ordinary proliferation assays.