MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana
MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana
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DOI：
10.1105/tpc.107.055855
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发表时间：
2008-05-01
期刊：
影响因子：
11.6
通讯作者：
Yoshioka, Hirofumi
中科院分区：
文献类型：
--
作者：
Asai, Shuta;Ohta, Kohji;Yoshioka, Hirofumi
一氧化氮（NO）和活性氧（ROS）是植物天然免疫的信号分子。自由基爆发由卵菌病原体致病疫霉产生的INF 1激发素诱导。NOA 1和NADPH氧化酶参与自由基爆发。在这里，我们表明，丝裂原活化蛋白激酶（MAPK）级联MEK 2-SIPK/NTF 4和MEK 1-NTF 6参与自由基爆发的调节。本氏烟草叶片中，SIPK/NTF 4的条件激活诱导NO的产生，而NTF 6不诱导NO的产生。INF 1和SIPK/NTF 4介导的NO爆发受到NOA 1敲低的影响。然而，ROS的产生是由SIPK/NTF 4或NTF 6诱导的。INF 1和MAPK介导的ROS生成通过沉默呼吸爆发氧化酶同源物B（RBOHB）（NADPH氧化酶的一种诱导型）来消除。在SIPK/NTF 4/NTF 6沉默的叶片中，INF 1诱导的RBOHB表达受损。这些结果表明，INF 1通过MAPK级联调节NOA 1介导的NO和RBOHB依赖的ROS产生。NOA 1沉默诱导对炭疽菌的高敏感性，但不诱导对致病疫霉的高敏感性;相反，RBOHB沉默降低对致病疫霉的抗性，但不诱导对炭疽菌的高敏感性。轮匝肌。这些结果表明，自由基爆发对植物-病原菌互作中防御反应的影响是多样的。
Nitric oxide (NO) and reactive oxygen species (ROS) act as signals in innate immunity in plants. The radical burst is induced by INF1 elicitin, produced by the oomycete pathogen Phytophthora infestans. NO ASSOCIATED1 (NOA1) and NADPH oxidase participate in the radical burst. Here, we show that mitogen-activated protein kinase (MAPK) cascades MEK2-SIPK/NTF4 and MEK1-NTF6 participate in the regulation of the radical burst. NO generation was induced by conditional activation of SIPK/NTF4, but not by NTF6, in Nicotiana benthamiana leaves. INF1- and SIPK/NTF4-mediated NO bursts were compromised by the knockdown of NOA1. However, ROS generation was induced by either SIPK/NTF4 or NTF6. INF1-and MAPK-mediated ROS generation was eliminated by silencing Respiratory Burst Oxidase Homolog B (RBOHB), an inducible form of the NADPH oxidase. INF1-induced expression of RBOHB was compromised in SIPK/NTF4/NTF6-silenced leaves. These results indicated that INF1 regulates NOA1-mediated NO and RBOHB-dependent ROS generation through MAPK cascades. NOA1 silencing induced high susceptibility to Colletotrichum orbiculare but not to P. infestans; conversely, RBOHB silencing decreased resistance to P. infestans but not to C. orbiculare. These results indicate that the effects of the radical burst on the defense response appear to be diverse in plant-pathogen interactions.