Mild experimental brain injury differentially alters the expression of neurotrophin and neurotrophin receptor mRNAs in the hippocampus
Mild experimental brain injury differentially alters the expression of neurotrophin and neurotrophin receptor mRNAs in the hippocampus
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DOI：
10.1006/exnr.1999.7216
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发表时间：
1999-12-01
影响因子：
5.3
通讯作者：
Seroogy, KB
中科院分区：
文献类型：
--
作者：
Hicks, RR;Martin, VB;Seroogy, KB
对轻度创伤性脑损伤后认知障碍的分子机制还知之甚少。神经营养因子，如脑源性神经营养因子（BDNF），已被确定为具有在学习和记忆中的作用。我们以前已经证明，中度（2.0-2.1大气压）的实验性脑外伤后，BDNF及其高亲和力受体trkB的mRNA水平在海马中双侧增加数小时，而NT-3 mRNA表达下降。在本研究中，我们使用原位杂交比较BDNF，trkB，NT-3，和trkC mRNA表达在大鼠海马在3或6小时后，侧部液压冲击脑损伤（FPI）的严重程度（1.0大气压）假损伤的对照组在相同的时间点。轻度FPI诱导海马中BDNF和trkB mRNA的杂交水平显著增加，NT-3 mRNA减少。然而，与中度实验性脑损伤的双侧效应相反，轻度损伤的变化仅限于损伤侧。这些发现表明，即使是轻微的创伤性脑损伤也会不同程度地改变海马中神经营养因子和神经营养因子受体的水平。这种改变可能对轻度脑损伤患者的神经可塑性和功能恢复具有重要意义。(C)北京：科学出版社.
The molecular events responsible for impairments in cognition following mild traumatic brain injury are poorly understood. Neurotrophins, such as brain-derived neurotrophic factor (BDNF), have been identified as having a role in learning and memory. We have previously demonstrated that following experimental brain trauma of moderate severity (2.0-2.1 atm), mRNA levels of BDNF and its high-affinity receptor, trkB, are increased bilaterally in the hippocampus for several hours, whereas NT-3 mRNA expression is decreased. In the present study, we used in situ hybridization to compare BDNF, trkB, NT-3, and trkC mRNA expression in rat hippocampus at 3 or 6 h after a lateral fluid percussion brain injury (FPI) of mild severity (1.0 atm) to sham-injured controls at equivalent time points. Mild FPI induced significant increases in hybridization levels for BDNF and trkB mRNAs, and a decrease in NT-3 mRNA in the hippocampus. However, in contrast to the bilateral effects of moderate experimental brain injury, the present changes with mild injury were restricted to the injured side. These findings demonstrate that even a mild traumatic brain injury differentially alters neurotrophin and neurotrophin receptor levels in the hippocampus. Such alterations may have important implications for neural plasticity and recovery of function in people who sustain a mild head injury. (C) 1999 Academic Press.