Synthesis and characterization of molecularly imprinted polymers for detection of the local anesthetic lidocaine in urine
Synthesis and characterization of molecularly imprinted polymers for detection of the local anesthetic lidocaine in urine
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DOI：
10.1002/sscp.202200081
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发表时间：
2022-11-24
影响因子：
1.1
通讯作者：
Jin, Jiye
中科院分区：
文献类型：
--
作者：
Takahashi, Fumiki;Matsuda, Kazusane;Jin, Jiye
分子印迹聚合物是一种可以利用分子识别捕获目标分析物的材料。这些聚合物可以有效地检测含有各种基质成分的生物样品（如尿液和血液）中的痕量目标化学物质。我们的目的是分析分子印迹聚合物与靶分子利多卡因的相互作用，利多卡因是一种氨基酰胺药物。采用本体聚合法制备了以利多卡因为模板的分子印迹聚合物。气相色谱-质谱分析表明，利多卡因与分子印迹聚合物中的识别位点有很强的相互作用。用Scatchard分析证实了分子印迹聚合物对利多卡因的非选择性吸附。利用两种结构相似的麻醉剂普鲁卡因和丁卡因结合强度的差异，优化了洗涤液分离利多卡因的条件。分子印迹聚合物能吸附尿样品中的利多卡因，不受干扰物的影响。因此，它们可以应用于生物材料的分析。这些结果表明，分子印迹聚合物可用于高选择性的分离和检测，可用于法医和临床分析。
Molecularly imprinted polymers are materials that can capture target analytes using molecular recognition. These polymers can efficiently detect trace target chemical species in biological samples, such as urine and blood, containing various matrix components. We aimed to analyze the interaction of molecularly imprinted polymers with the target molecule lidocaine, which is an amino amide pharmaceutical. Lidocaine-templated molecularly imprinted polymers were synthesized by bulk polymerization. Gas chromatography-mass spectrometry showed there was a strong interaction between lidocaine and the recognition site in the molecularly imprinted polymers. Non-selective adsorption of lidocaine on the molecularly imprinted polymers was confirmed and characterized by Scatchard analysis. The washing solution was optimized for the separation of lidocaine from the structurally similar anesthetics procaine and tetracaine by taking advantage of differences in binding strength. The molecularly imprinted polymers could adsorb lidocaine in urine samples and were not affected by interfering compounds. Consequently, they could be applied to the analysis of biological materials. These results suggest that the molecularly imprinted polymers can be used for separation and detection with high selectivity and could be applied in forensic and clinical analysis.