Interleukin-1 alpha alters the expression of matrix metalloproteinases and collagen degradation by pulp fibroblasts.
Interleukin-1 alpha alters the expression of matrix metalloproteinases and collagen degradation by pulp fibroblasts.
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Interleukin-1 α 改变基质金属蛋白酶的表达和牙髓成纤维细胞的胶原蛋白降解。
DOI：
10.1016/j.joen.2005.10.055
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Windsor,LJack
中科院分区：
文献类型：
--
作者：
Wisithphrom,Kessiri;Murray,PeterE;Windsor,LJack
基质金属蛋白酶（MMPs）和基质金属蛋白酶组织抑制剂（TIMPs）已被认为在牙髓破坏中发挥作用。本研究探讨白细胞介素（IL）-1 α对牙髓成纤维细胞的影响。使用细胞介导的胶原降解试验，在有或没有IL-1 α的情况下测定这些细胞降解胶原的能力。采用逆转录-聚合酶链反应（RT-PCR）检测IL-1 α对多种MMPs和TIMPs mRNA表达的影响，Western blot和酶谱分析检测其蛋白表达。这些细胞介导的胶原降解可被IL-1 α刺激，并被MMP抑制剂抑制。IL-1 α可诱导MMP-1、MMP-3 mRNA表达增加，并诱导MMP-7表达增加。Western blot分析证实了这些结果。IL-1 α可增加TIMP-1的分泌水平，而对TIMP-2的分泌水平影响不大。提示IL-1 α可能通过不同程度地调节MMPs和TIMPs而引起牙髓损伤。
Matrix metalloproteinases (MMPs) and the tissue inhibitors of MMPs (TIMPs) have been suggested to play a role in dental pulp destruction. This study examined the effects of interleukin (IL)-1 α on pulp fibroblasts. The ability of these cells to degrade collagen was determined with or without IL-1 α utilizing a cell-mediated collagen degradation assay. Reverse transcriptase-polymerase chain reaction was utilized to examine the mRNA expression of multiple MMPs and TIMPs with and without IL-1 α, while Western blot analyses and zymography were utilized to examine their protein expression. The collagen degradation mediated by these cells was stimulated by IL-1 α and inhibited by MMP inhibitors. IL-1 α increased the mRNA expression of MMP-1 and MMP-3, as well as induced MMP-7. Western blot analyses confirmed these results. IL-1 α increased the secreted protein level of TIMP-1, while only slightly affected the level of TIMP-2. These results suggest that IL-1 α can induce pulp destruction by differentially regulating MMPs and TIMPs.