ETHANOL INHIBITS HUMAN BONE CELL-PROLIFERATION AND FUNCTION-INVITRO
ETHANOL INHIBITS HUMAN BONE CELL-PROLIFERATION AND FUNCTION-INVITRO
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DOI：
10.1016/0026-0495(91)90044-w
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发表时间：
1991-06-01
影响因子：
9.8
通讯作者：
HOWARD, GA
中科院分区：
文献类型：
--
作者：
FRIDAY, KE;HOWARD, GA
在体外研究了乙醇对人骨细胞增殖和功能的直接影响。以骨小梁片为材料，采用胶原酶消化法制备正常人成骨细胞。将这些成骨细胞暴露于浓度为0.05%至1%的乙醇中22小时，通过掺入3h -胸腺嘧啶来评估骨细胞DNA合成的剂量依赖性减少。浓度为0.01%至1%的乙醇暴露72小时后，通过将3h -脯氨酸掺入三氯乙酸（TCA）可沉淀物质中测量的蛋白质合成以剂量依赖性方式减少。1%乙醇处理72小时后，人骨细胞蛋白浓度和碱性磷酸酶总活性显著降低，较低浓度乙醇处理无显著降低。这种成骨细胞增殖和活性的减少可能部分解释了摄入过量乙醇的人骨质减少的发生。
The direct effects of ethanol on human bone cell proliferation and function were studied in vitro. Normal human osteoblasts from trabecular bone chips were prepared by collagenase digestion. Exposure of these osteoblasts to ethanol in concentrations of 0.05% to 1% for 22 hours induced a dose-dependent reduction in bone cell DNA synthesis as assessed by incorporation of3H-thymidine. After 72 hours of ethanol exposure in concentrations of 0.01% to 1%, protein synthesis as measured by3H-proline incorporation into trichbroacetic acid (TCA)-precipitable material was reduced in a dose-dependent manner. Human bone cell protein concentrations and alkaline phosphatase total activity were significantly reduced after exposure to 1% ethanol for 72 hours, but not with lower concentrations of ethanol. This reduction in osteoblast proliferation and activity may partially explain the development of osteopenia in humans consuming excessive amounts of ethanol.