CARBONIC ANHYDRASE-IV ON BRAIN CAPILLARY ENDOTHELIAL-CELLS - A MARKER ASSOCIATED WITH THE BLOOD-BRAIN-BARRIER
CARBONIC ANHYDRASE-IV ON BRAIN CAPILLARY ENDOTHELIAL-CELLS - A MARKER ASSOCIATED WITH THE BLOOD-BRAIN-BARRIER
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DOI：
10.1073/pnas.89.15.6823
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发表时间：
1992-08-01
影响因子：
11.1
通讯作者：
SLY, WS
中科院分区：
文献类型：
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作者：
GHANDOUR, MS;LANGLEY, OK;SLY, WS
碳酸酐酶（CA）活性在控制脑脊液产生中起重要作用，也影响神经兴奋和对癫痫发作的易感性。直到最近，CA II是唯一在脑部发现的CA。其分布仅限于脉络膜丛的上皮细胞和髓鞘形成细胞，即少突胶质细胞。在本报告中，我们使用从大鼠肺中提取的CA - IV抗体进行免疫印迹，结果显示CA - IV也存在于大鼠和小鼠的大脑中。对大鼠和小鼠脑切片的免疫组织化学和免疫电镜结果显示，CA IV的分布与CA II的分布有很大的不同。caiv表达于脑毛细血管内皮细胞的管腔表面，且仅限于管腔表面。这些结果证实了CA IV是与血脑屏障相关的细胞化学标记物，并表明CA IV在脑内CO2和HCO3-稳态中起重要作用。
Carbonic anhydrase (CA) activity plays an important role in controlling cerebrospinal fluid production and also influences neuroexcitation and susceptibility to seizures. Until recently, CA II was the only CA demonstrated in brain. Its distribution is limited to the epithelial cells of the choroid plexus and to the myelin-forming cells, the oligodendrocytes. In this report, we present immunoblots, using an antibody raised to CA IV from rat lung, that show that CA IV is also present in rat and mouse brain. Results of immunohistochemistry and immunoelectron microscopy on sections from rat and mouse brain are presented that show the distribution of CA IV to be quite distinct from that of CA II. CA IV is expressed on and is limited to the luminal surface of endothelial cells of cerebral capillaries. These results establish CA IV as a cytochemical marker associated with the blood-brain barrier and suggest an important role for CA IV in CO2 and HCO3- homeostasis in brain.