Evolutionary history of Caenorhabditis elegans inferred from microsatellites:: Evidence for spatial and temporal genetic differentiation and the occurrence of outbreeding
Evolutionary history of Caenorhabditis elegans inferred from microsatellites:: Evidence for spatial and temporal genetic differentiation and the occurrence of outbreeding
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DOI：
10.1093/molbev/msh264
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发表时间：
2005-01-01
影响因子：
10.7
通讯作者：
Schulenburg, H
中科院分区：
文献类型：
--
作者：
Haber, M;Schüngel, M;Schulenburg, H
虽然不同的生物学科采用线虫秀丽隐杆线虫作为一个高效的实验室模型系统，很少有人知道它的自然历史。我们研究了它的进化历史，使用10个多态性三核苷酸和四核苷酸微卫星，来自整个基因组。这些微卫星分析了来自世界不同地区的35个先前可用的天然分离株以及从德国西北部分离的23个新菌株。我们的研究结果表明，C.秀丽线虫的谱系在遗传上的差异与地理距离有关，并且在较小程度上与菌株分离时间的差异有关。后者表明在特定位置菌株基因型的一些周转。我们的数据还表明，高度多样化的基因型共存的人口从西北德国，这是最好的解释最近的迁移事件。此外，自交被确认为该两性线虫在自然界中的主要繁殖方式。重要的是，我们还发现了偶尔发生远系繁殖的证据。综上所述，这些结果支持了先前的观点，即C。秀丽隐杆线虫是一种殖民者，因此即使在没有潜在配偶的情况下，出售也可能允许在新的栖息地内迅速扩散，而偶尔的异交可能有助于弥补近亲繁殖的缺点。这些关于C. elegans的研究对于深入了解这种生物的复杂性，包括其多方面的发育，神经或分子遗传途径具有重要价值。
Although diverse biological disciplines employ the nematode Caenorhabditis elegans as a highly efficient laboratory model system, little is known about its natural history. We investigated its evolutionary past using 10 polymorphic trinucleotide and tetranucleotide microsatellites, derived from across the whole genome. These microsatellites were analyzed from the 35 previously available natural isolates from different parts of the world and also 23 new strains isolated from northwest Germany. Our results highlight that C. elegans lineages differentiate genetically with respect to geographic distance and, to a lesser extent, differences in the time of strain isolation. The latter indicates some turnover of strain genotypes at specific locations. Our data also demonstrate the coexistence of highly diverse genotypes in the population from northwest Germany, which is best explained by recent migration events. Furthermore, selfing is confirmed as the primary mode of reproduction for this hermaphroditic nematode in nature. Importantly, we also find evidence for the occurrence of occasional outbreeding. Taken together, these results support the previous notion that C. elegans is a colonizer, whereby selling may permit rapid dispersal within new habitats even in the absence of potential mates, whereas occasional outcrossing may serve to compensate for the disadvantages of inbreeding. Such information about the natural history of C. elegans should be of great value for an in-depth understanding of the complexity of this organism, including its multifaceted developmental, neurological, or molecular genetic pathways.