Characterization of stipe elongation of the mushroom Coprinopsis cinerea.
Characterization of stipe elongation of the mushroom Coprinopsis cinerea.
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DOI：
10.1099/mic.0.079418-0
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发表时间：
2014-09
期刊：
影响因子：
1.5
通讯作者：
Wenming Zhang;Xiuxiu Wu;Yajun Zhou;Zhonghua Liu;W. Zhang;Xin Niu;Yan Zhao;Siyu Pei;Yang Zhao;Sheng Yuan
中科院分区：
文献类型：
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作者：
Wenming Zhang;Xiuxiu Wu;Yajun Zhou;Zhonghua Liu;W. Zhang;Xin Niu;Yan Zhao;Siyu Pei;Yang Zhao;Sheng Yuan
以前，我们观察到金针菇顶端柄在从中性到酸性的pH变化后的15分钟内，酸诱导的短时壁延长。这种酸诱导的柄壁伸展被加热消除，并由蜗牛Expansin样蛋白重组，尽管我们未能从鹅绒委陵菜中分离到任何内源Expansin样蛋白，因为其有限的1 mm快速伸长区域。在这项研究中，我们报道了鸡冠花柄具有9 mm的快速伸长顶端区域，这是适合作为壁延展研究的模型材料。伸长的顶端柄在酸诱导下有两个伸壁阶段，在最初的15min内发生一次快速的短壁伸展，在随后的2 h内发生缓慢的、逐渐衰退的长壁伸展。在加热或蛋白失活处理后，顶柄失去了长期的伸壁，而保留了较慢的短壁伸展，该伸展是由一种类似Exansin的蜗牛蛋白重组的。相反，非伸长的基柄仅表现出较弱的短期壁延伸。我们认为，长期的壁伸长是一个参与柄伸长的蛋白质介导的过程，而短期的壁伸长是一个非蛋白质介导的过程，与柄的伸长无关。
Previously, we observed an acid-induced short-term wall extension in Flammulina velutipes apical stipes during a 15 min period after a change from a neutral to an acidic pH. This acid-induced stipe wall extension was eliminated by heating and reconstituted by a snail expansin-like protein, although we failed to isolate any endogenous expansin-like protein from F. velutipes because of its limited 1 mm fast elongation region. In this study, we report that Coprinopsis cinerea stipes possess a 9 mm fast elongation apical region, which is suitable as a model material for wall extension studies. The elongating apical stipe showed two phases of acid-induced wall extension, an initial quick short-term wall extension during the first 15 min and a slower, gradually decaying long-term wall extension over the subsequent 2 h. After heating or protein inactivation pretreatment, apical stipes lost the long-term wall extension, retaining a slower short-term wall extension, which was reconstituted by an expansin-like snail protein. In contrast, the non-elongating basal stipes showed only a weaker short-term wall extension. We propose that the long-term wall extension is a protein-mediated process involved in stipe elongation, whereas the short-term wall extension is a non-protein mediated process not involved in stipe elongation.