Cholesteryl group- and acryloylgroup-bearing pullulan nanogel to deliver BMP2 and FGF18 for bone tissue engineering
Cholesteryl group- and acryloylgroup-bearing pullulan nanogel to deliver BMP2 and FGF18 for bone tissue engineering
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含有胆固醇基团和丙烯酰基基团的支链淀粉纳米凝胶可提供用于骨组织工程的 BMP2 和 FGF18
DOI：
10.1111/j.1600-0765.2011.01419.x
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发表时间：
2012
期刊：
影响因子：
14
通讯作者：
Iseki S
中科院分区：
文献类型：
--
作者：
Fujioka-Kobayashi M;Ota MS;Shimoda A;Nakahama K;Akiyoshi K;Miyamoto Y;Iseki S
Date Y，Yokoyama Y，Kondo H，黑田S，Ohya K，Ota MS，Iseki S，春日井S.染色质重塑相关因子Chd 3在牙根发育过程中的表达J Periodont Res 2012; 47：180-187.© 2011 John Wiley & Sons A/S背景和目的：牙根是维持牙齿功能的关键部位之一，然而牙根发育的分子机制仍不清楚。我们的目的是确定特定因素的根形态发生使用一个新开发的实验system.Material和方法：暂定成牙骨质细胞和牙周膜细胞从小鼠下颌磨牙分离，采用激光捕获显微切割。分别捕获了500多个成牙骨质细胞和牙周膜细胞。RNA提取和扩增后，通过cDNA微阵列分析，在分离的成牙骨质细胞和牙周膜细胞的mRNA表达进行了比较。然后，假定的成牙骨质细胞特异性基因进行原位杂交分析，以确认在小鼠下颌骨的结果。其中，锌指解旋酶（ZFH），也称为染色体结构域解旋酶DNA结合蛋白3（Chd 3），是暂时性成牙骨质细胞中高表达的转录本之一。原位杂交显示ZFH/Chd 3在Hertwig的上皮根鞘中强烈表达，而不是在牙骨质中。 而且，当牙根在第一磨牙中形成时，其表达消失。结论：ZFH/Chd 3在牙根发育和牙骨质形成过程中起重要作用。
Date Y, Yokoyama Y, Kondo H, Kuroda S, Ohya K, Ota MS, Iseki S, Kasugai S. Restricted expression of chromatin remodeling associated factorChd3during tooth root development. J Periodont Res 2012; 47: 180–187. © 2011 John Wiley & Sons A/SBackground and Objective:The tooth root is one of the critical parts to maintain tooth function; however, the molecular mechanisms of root development remain unknown. We aimed to identify specific factors for root morphogenesis using a newly developed experimental system.Material and Methods:Tentative cementoblasts and periodontal ligament cells from mouse mandibular molars were isolated using laser capture microdissection. More than 500 cementoblasts and periodontal ligament cells were separately captured. After RNA extraction and amplification, mRNA expression in isolated cementoblasts was compared with that of periodontal ligament cells by cDNA microarray analysis. Then, putative cementoblast‐specific genes were subjected toin situhybridization analysis to confirm the results in mouse mandible.Results:Approximately 2000 genes were differentially expressed between these tissues. Among those genes,zinc finger helicase (ZFH), also termedchromodomain‐helicase‐DNA‐binding protein 3 (Chd3), was one of the highly expressed transcripts in tentative cementoblasts.In situhybridization revealed thatZFH/Chd3was strongly expressed in Hertwig’s epithelial root sheath rather than in cementum. Moreover, its expression disappeared when root formation was advanced in the first molar. In contrast,Chd3was continuously expressed in dental epithelial cells of the cervical loop, in which root extension is never terminated.Conclusion:These results suggest thatZFH/Chd3might play an important role in tooth root development and subsequent cementogenesis.