Shovelomics: high throughput phenotyping of maize (Zea mays L.) root architecture in the field
Shovelomics: high throughput phenotyping of maize (Zea mays L.) root architecture in the field
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DOI：
10.1007/s11104-010-0623-8
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发表时间：
2011-04-01
期刊：
影响因子：
4.9
通讯作者：
Lynch, Jonathan P.
中科院分区：
文献类型：
--
作者：
Trachsel, Samuel;Kaeppler, Shawn M.;Lynch, Jonathan P.
我们提出了一种方法，以视觉评分10根冠的成年玉米植株在几分钟内在外地的根构型性状。对3个玉米重组自交系群体进行了表型分析。2008年在宾夕法尼亚州的粉壤土和威斯康星州的桑迪土壤中进行，2009年在宾夕法尼亚州再次进行。在开花时，目视评估冠根和支撑根的数量、角度和分枝模式。根据植物生长的土壤类型，样品处理时间从3分钟（沙子）到8分钟（粉砂壤土）不等。无论环境如何，每个样品的牙冠目视测量需要2分钟。根冠的视觉评分给出了根构型特征值的可靠估计，如支撑根和冠根的数量（r（2）= 0.46-0.97）、角度（r（2）= 0.66-0.76）和分支（r（2）= 0.54-0.88）的测量和视觉评分特征值之间的高度相关性所示。基于视觉评价的根冠性状，有可能区分种群。来自杂交NY 821 × H99的RILs通常具有最大的根数、最高的分枝密度和最浅的根角，而来自杂交OH 43 × W 64 a的自交系通常具有最陡的根角。基因型的排名在不同环境中保持不变，强调了该方法在不同环境中评估基因型的适用性。与冠根相比，支具根的评分与实际测量值的相关性更好。根构型的可视化评价将是一种有价值的工具，在剪裁作物根系系统，以特定的环境。
We present a method to visually score 10 root architectural traits of the root crown of an adult maize plant in the field in a few minutes. Phenotypic profiling of three recombinant inbred line (RIL) populations of maize (Zea mays L.; B73xMo17, Oh43xW64a, Ny821xH99) was conducted in 2008 in a silt loam soil in Pennsylvania and in a sandy soil in Wisconsin, and again in 2009 in Pennsylvania. Numbers, angles and branching pattern of crown and brace roots were assessed visually at flowering. Depending on the soil type in which plants were grown, sample processing took from three (sand) to 8 min (silt-loam). Visual measurement of the root crown required 2 min per sample irrespective of the environment. Visual scoring of root crowns gave a reliable estimation of values for root architectural traits as indicated by high correlations between measured and visually scored trait values for numbers (r (2) = 0.46-0.97), angles (r (2) = 0.66-0.76), and branching (r (2) = 0.54-0.88) of brace and crown roots. Based on the visual evaluation of root crown traits it was possible to discriminate between populations. RILs derived from the cross NY821 x H99 generally had the greatest number of roots, the highest branching density and the most shallow root angles, while inbred lines from the cross between OH43 x W64a generally had the steepest root angles. The ranking of genotypes remained the same across environments, emphasizing the suitability of the method to evaluate genotypes across environments. Scoring of brace roots was better correlated with the actual measurements compared to crown roots. The visual evaluation of root architecture will be a valuable tool in tailoring crop root systems to specific environments.