SERS and fluorescence dual-mode sensing trace hemin and K+ based on Gquarplex/hemin DNAzyme catalytic amplification
SERS and fluorescence dual-mode sensing trace hemin and K+ based on Gquarplex/hemin DNAzyme catalytic amplification
复制标题
基于 Gquarplex/hemin DNAzyme 催化扩增的 SERS 和荧光双模式传感痕量血红素和 K
DOI：
10.1016/j.snb.2019.126799
复制
发表时间：
2019-10-15
影响因子：
8.4
通讯作者：
Jiang, Zhiliang
中科院分区：
文献类型：
--
作者：
Li, Dan;Li, Chongning;Jiang, Zhiliang
K ~+的适体（Apt）是富含鸟嘌呤G的核苷酸序列，Apt能与K ~+和氯化血红素（HM）结合形成稳定的G-四链体/HM DNA酶，具有很强的催化活性。在超声辐射下，DNAzyme催化H2 O2氧化四甲基联苯胺（TMB）生成具有荧光效应和表面增强拉曼光谱（Sers）活性的氧化产物TMBOX。在优化的分析条件下，HM和K+的Sers检测线性范围分别为0.01 ~ 75 nmol/L和2-1000 nmol/L，检测限分别为0.004 nmol/L和1.6 nmol/L。HM和K+的荧光线性范围分别为1-100 nmol/L和10-300 nmol/L，检出限分别为0.88 nmol/L和9.4 nmol/L。因此，建立了一个Sers和荧光双模式平台，用于痕量HM和K+的测定。
The aptamer of K+ (Apt) was a guanine G-rich nucleotide sequence, Apt can bind K+ and hemin (HM) to form a stable G-quadruplex/HM DNAzyme with strong catalytic activity. Under ultrasonic irradiation, DNAzyme catalyzed the oxidation of tetramethylbenzidine (TMB) by H2O2 to form oxidation product TMBOX with fluorescence effect and SERS activity. Under the optimized analytical conditions, the linear range of catalyzed SERS determination of HM and K+ were 0.01-75 nmol/L and 2-1000 nmol/L, with the detection limits of 0.004 nmol/L and 1.6 nmol/L, respectively. The fluorescence linear ranges of HM and K+ were 1-100 nmol/L and 10-300 nmol/L, with the detection limits of 0.88 nmol/L and 9.4 nmol/L, respectively. Thus, a SERS and fluorescence dual-mode platform was developed for determination of trace HM and K+.