DNA-polyfluorophore Chemosensors for Environmental Remediation: Vapor-phase Identification of Petroleum Products in Contaminated Soil.
DNA-polyfluorophore Chemosensors for Environmental Remediation: Vapor-phase Identification of Petroleum Products in Contaminated Soil.
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DOI：
10.1039/c3sc50985k
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发表时间：
2013-08-01
期刊：
影响因子：
8.4
通讯作者：
Kool ET
中科院分区：
文献类型：
--
作者：
Jiang W;Wang S;Yuen LH;Kwon H;Ono T;Kool ET
石油产品对土壤和地下水的污染是一个极其普遍和重要的环境问题。在这里，我们测试了一种简单的光学方法来检测和识别土壤样本中的工业污染物，使用一组基于模式的荧光dna化学传感器。我们使用了一组不同的工业挥发性化学物质来筛选和鉴定peg -聚苯乙烯微珠上的一组五短寡聚DNA荧光团，这些荧光团在暴露于空气中的蒸汽后可以区分整个组。然后，我们测试了这组五种荧光化学传感器化合物在暴露于被原油、石油馏分油、燃料、润滑剂和添加剂等十种不同样品中的一种污染的土壤样品上的headgas时对荧光变化的响应能力。定量荧光变化数据（如Δ（R，G，B）发射强度）的统计分析表明，这五种化学传感器可以在30分钟内区分土壤中1000ppm的所有十种产品混合物。对其中三种污染物混合物的敏感性测试表明，它们在土壤中的含量至少低至百万分之一，可以被检测和区分。结果表明，dna -多荧光团在监测环境溢漏和泄漏发生时和补救期间的程度和特征方面可能具有实际效用。
Contamination of soil and groundwater by petroleum-based products is an extremely widespread and important environmental problem. Here we have tested a simple optical approach for detecting and identifying such industrial contaminants in soil samples, using a set of fluorescent DNA-based chemosensors in pattern-based sensing. We used a set of diverse industrial volatile chemicals to screen and identify a set of five short oligomeric DNA fluorophores on PEG-polystyrene microbeads that could differentiate the entire set after exposure to their vapors in air. We then tested this set of five fluorescent chemosensor compounds for their ability to respond with fluorescence changes when exposed to headgas over soil samples contaminated with one of ten different samples of crude oil, petroleum distillates, fuels, lubricants and additives. Statistical analysis of the quantitative fluorescence change data (as Δ(R,G,B) emission intensities) revealed that these five chemosensors on beads could differentiate all ten product mixtures at 1000 ppm in soil within 30 minutes. Tests of sensitivity with three of the contaminant mixtures showed that they could be detected and differentiated in amounts at least as low as one part per million in soil. The results establish that DNA-polyfluorophores may have practical utility in monitoring the extent and identity of environmental spills and leaks, while they occur and during their remediation.