Examination of the effect of combined use of Er:YAG laser irradiation and mechanical force loading on bone metabolism using primary human gingival fibroblasts
Examination of the effect of combined use of Er:YAG laser irradiation and mechanical force loading on bone metabolism using primary human gingival fibroblasts
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使用原代人牙龈成纤维细胞检查联合使用 Er:YAG 激光照射和机械力加载对骨代谢的影响
DOI：
10.1007/s10103-020-03079-y
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Tanimoto K.
中科院分区：
文献类型：
--
作者：
Tsuka Y;Kunimatsu R;Gunji H;Abe T;Medina CC;Hiraki T;Nakatani A;Sakata S;Rikitake K;Aisyah PN;Hirose N;Yanosita M;Tanimoto K.
长时间的治疗和疼痛的牙齿移动是患者接受正畸治疗的主要问题。加速牙齿移动会导致治疗周期的缩短，因此在正畸领域对牙齿移动进行了各种研究。在以前的研究中，我们使用Er：YAG激光对大鼠进行了纤维切开式纤维切开术，并证实了牙齿移动的加速。因此，本研究在体外研究了Er：YAG激光照射对人牙龈成纤维细胞的影响。将人牙龈成纤维细胞(2.0 × 10 5细胞)接种于6孔板中，2 4h后达到80%融合。对照组不接受任何照射，3组分别接受0.6W、1.0W和1.2W的激光照射。细胞接种后24小时行激光照射。24小时后细胞复苏，用聚合酶链式反应检测环氧合酶-2(COX-2)、白介素1β(IL-1β)、肿瘤坏死因子-α(TN-α)、骨形态发生蛋白-2(BMP-2)和骨形态发生蛋白-4(BMP-4)基因的表达。另设对照组、单纯Er：YAG激光照射组、单纯离心力加载组、Er：YAG激光照射+离心力加载组。24小时后收集细胞，进行聚合酶链式反应。激光照射后24小时，定量RT-PCR法检测COX-2、IL-1β、肿瘤坏死因子-α、骨形态发生蛋白-2和骨形态发生蛋白-4的基因表达随照射能量的增加而增加，以1.2W为最大值，激光离心负荷组COX-2、IL-1β、肿瘤坏死因子-α、骨形态发生蛋白-2和骨形态发生蛋白-4的基因表达明显高于负荷组。这些结果表明，当机械刺激和激光照射相结合时，人牙龈成纤维细胞中与骨代谢相关的基因被激活。这有助于阐明Er：YAG激光照射在牙齿移动过程中的作用。
Prolonged treatment and painful tooth movement are major problems for patients undergoing orthodontic treatment. Accelerating the movement of teeth leads to shortening of the treatment period, so various studies on the movement of teeth have been conducted in the field of orthodontics. In previous studies, we performed a fiber incision-like fiberotomy using an Er:YAG laser in rats and confirmed acceleration of tooth movement. Therefore, in this study, the effect of Er:YAG laser irradiation on human gingival fibroblasts was investigated in vitro. Human gingival fibroblasts (2.0 × 105cells) were seeded in a 6-well plate and reached 80% confluence 24 h later. A control group not undergoing any irradiation and 3 groups undergoing laser irradiation at 0.6 W, 1.0 W, and 1.2 W were investigated. Laser irradiation was performed 24 h after cell seeding. The cells were then recovered 24 h later, and the cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), bone morphogenetic protein-2 (BMP-2), and BMP-4 genes were confirmed by PCR. In addition, a control group not undergoing any procedures, a group undergoing only Er:YAG laser irradiation, a group undergoing only centrifugal loading, and a group undergoing both Er:YAG laser irradiation and centrifugal force loading were investigated. After 24 h, cells were collected and PCR was performed. Twenty-four hours after laser irradiation, gene expressions were examined by quantitative RT-PCR, which showed that the gene expressions of COX-2, IL-1β, TNF-α, BMP-2, and BMP-4 increased depending on the amount of irradiation energy, with the largest value at 1.2 W. Gene expressions of COX-2, IL-1β, TNF-α, BMP-2, and BMP-4 were significantly higher in the laser with centrifugal load group than in the load group. These results suggest that genes related to bone metabolism are activated in human gingival fibroblasts when mechanical stimulation and laser irradiation are combined. This helps to elucidate the effects of Er:YAG laser irradiation during tooth movement.
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