Biofilm Production and Antibiofilm Activity of Echinocandins and Liposomal Amphotericin B in Echinocandin-Resistant Yeast Species
Biofilm Production and Antibiofilm Activity of Echinocandins and Liposomal Amphotericin B in Echinocandin-Resistant Yeast Species
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棘白菌素和脂质体两性霉素 B 在棘白菌素抗性酵母菌中的生物膜产生和抗菌膜活性
DOI：
10.1128/aac.03065-15
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发表时间：
2016
影响因子：
4.9
通讯作者：
J. Guinea
中科院分区：
文献类型：
--
作者：
L. Marcos;Marta Gomez;P. Escribano;Ó. Zaragoza;E. Bouza;J. Guinea
摘要棘球菌素和两性霉素B脂质体对棘球菌素敏感的念珠菌生物被膜有一定的抑制作用。然而，有关棘球绦虫耐药株生物被膜的产生及其对真菌的敏感性的报道很少。我们研究了FKS突变的念珠菌和对棘球菌素耐药的非念珠菌的生物被膜的产生，以及这两种菌株对脂质体两性霉素B和棘球菌素的敏感性。我们分析了真菌血症患者分离的菌株(FKS突变株念珠菌，n=5；内在棘球菌素耐药的非念珠菌，n=12；念珠菌野生型，n=10)的生物被膜产生情况。根据生物量(结晶紫试验)和代谢活性(XTT还原试验)对菌株进行生物被膜形成分类。将预制生物膜与脂质体两性霉素B、卡泊芬净、米卡芬净和阿尼达芬净进行对比试验。固着MIC的定义是，与生长对照(分别为SMIC50和SMIC80)相比，产生生物膜代谢活性下降50%或80%的抗真菌浓度。FKS突变株形成生物膜的方式与念珠菌野生型菌株相似。棘球菌素对野生型念珠菌形成的生物被膜的抑制活性最高，其次是FKS突变型念珠菌和非念珠菌。两性霉素B脂质体对FKS突变型念珠菌生物被膜的抑制作用最强。棘球菌素耐药株的生物被膜形成与野生型株相似，但对棘球菌素的耐药性仍然很高。
ABSTRACT The echinocandins and liposomal amphotericin B are active against biofilm produced by echinocandin-susceptible Candida strains. However, few data have been reported on the production of biofilm by echinocandin-resistant isolates and their antifungal susceptibility. We studied the production of biofilm by fks mutant Candida strains and intrinsically echinocandin-resistant non-Candida isolates and the susceptibility of both entities to liposomal amphotericin B and echinocandins. We analyzed the production of biofilm by isolates from patients with fungemia (fks mutant Candida, n = 5; intrinsically echinocandin-resistant non-Candida, n = 12; and Candida wild type, n = 10). Biofilm formation was measured to classify strains according to biomass (crystal violet assay) and metabolic activity (XTT reduction assay). Preformed biofilms were tested against liposomal amphotericin B, caspofungin, micafungin, and anidulafungin. The sessile MIC was defined as the antifungal concentration yielding a 50% or 80% reduction in the metabolic activity of the biofilm compared to that of the growth control (SMIC50 and SMIC80, respectively). fks mutant Candida isolates formed biofilms in a fashion similar to that of Candida wild-type strains. The echinocandins had the highest activity against biofilms formed by wild-type Candida isolates, followed by fks mutant Candida isolates and non-Candida isolates. Liposomal amphotericin B had the highest activity against fks mutant Candida biofilms. The formation of biofilm by echinocandin-resistant strains was similar to that of wild-type strains, although resistance to echinocandins remained high.
影响因子：
11.8
作者：
Alexander, Barbara D.;Johnson, Melissa D.;Pfaller, Michael A.
通讯作者：
Pfaller, Michael A.
抗棘白菌素白色念珠菌临床分离株形成的生物膜中矛盾的抗真菌活性和结构观察。
影响因子：
2.9
作者：
Walraven,CarlaJ;Bernardo,StellaM;Wiederhold,NathanP;Lee,SamuelA
通讯作者：
Lee,SamuelA
影响因子：
1.2
作者：
O'Toole, George A.
通讯作者：
O'Toole, George A.