EXPRESSION OF THE TIGHT JUNCTION PROTEIN ZO-1 IN THE OLFACTORY SYSTEM - PRESENCE OF ZO-1 ON OLFACTORY SENSORY NEURONS AND GLIAL-CELLS
EXPRESSION OF THE TIGHT JUNCTION PROTEIN ZO-1 IN THE OLFACTORY SYSTEM - PRESENCE OF ZO-1 ON OLFACTORY SENSORY NEURONS AND GLIAL-CELLS
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DOI：
10.1002/cne.903410402
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发表时间：
1994-03-22
影响因子：
2.5
通讯作者：
DERMIETZEL, R
中科院分区：
文献类型：
--
作者：
MIRAGALL, F;KRAUSE, D;DERMIETZEL, R
嗅觉系统是中枢神经系统的一个独特部分，因为它在成年后保留了神经元的更新和再生能力。这为研究细胞间相互作用中膜部分的可塑性提供了一个理想的模型。一种特别涉及细胞-细胞相互作用的结构是紧密连接，其建立上皮细胞的极化并产生细胞旁通道的扩散屏障。ZO-1是一种与紧密连接相关的磷蛋白。我们研究了ZO-1蛋白在发育和成年小鼠嗅觉系统中的表达，以获得有关该紧密连接组分的定位和发育表达的信息。ZO-1在嗅球细胞培养物中的表达也已被确定，ZO-1在紧密连接形成之前就存在于嗅基板中。ZO-1定位于发育和成熟的嗅上皮中的支持细胞和嗅神经元之间的异型接触，以及在这两种细胞类型之间的同型接触。共聚焦显微镜显示ZO-1的表达在不同的嗅树突之间的定量差异。在嗅神经ZO-1免疫标记可检测到嗅鞘细胞之间。从出生后第七天ZO-1免疫标记检测到在二尖瓣细胞层的灯泡上的细胞初步确定为少突胶质细胞。球的有髓纤维束呈ZO-1阴性。嗅球细胞培养显示ZO-1免疫反应，主要定位于胶质细胞酸性蛋白（GFAP）阳性细胞。我们的研究结果提供了进一步的证据，ZO-1的服务功能无关的紧密连接复合物，并表明这些细胞间接触的分子异质性。(C)1994 Wiley-Liss，Inc.
The olfactory system is a unique part of the central nervous system since it retains neuronal turnover and regenerative capacities in adulthood. Thus it provides an ideal model to study plasticity of membrane moities involved in cell-cell interactions. One structure particularly involved in cell-cell interaction is the tight junction, which establishes polarization of epithelial cells and creates diffusion barriers to paracellular passages. ZO-1 is a phosphoprotein peripherally associated with tight junctions. We have studied expression of ZO-1 protein in the developing and adult olfactory system of the mouse in order to get information about the localization and developmental expression of this tight junction component. ZO-1 expression has also been determined in cell cultures of olfactory bulbs.ZO-1 was present in the olfactory placode prior to formation of tight junctions. ZO-1 was localized in the developing and mature olfactory epithelium at heterotypic contacts between supporting cells and olfactory neurons as well as at homotypic contacts between both these cell types. Confocal microscopy showed quantitative differences in the ZO-1 expression among different olfactory dendrites. In the olfactory nerves ZO-1 immunolabeling was detectable between olfactory ensheathing cells. From the seventh postnatal day ZO-1 immunolabeling was detected at the mitral cell layer of the bulb on cells tentatively identified as oligodendrocytes. Myelinated tracts of the bulb were ZO-1 negative. Cell cultures of olfactory bulbs showed ZO-1 immunoreaction, mostly localized on glial fibrillary acidic protein (GFAP)-positive cells. Our results provide further evidence that ZO-1 serves functions unrelated to the tight junction complex and indicate molecular heterogeneity of these cell-cell contacts. (C) 1994 Wiley-Liss, Inc.