Two distinct succinate thiokinases in both bloodstream and procyclic forms of Trypanosoma brucei.
Two distinct succinate thiokinases in both bloodstream and procyclic forms of Trypanosoma brucei.
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布氏锥虫的血流和前循环形式中存在两种不同的琥珀酸硫激酶。
DOI：
10.1016/0006-291x(88)90587-6
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发表时间：
1988
影响因子：
3.1
通讯作者：
P. Weitzman
中科院分区：
文献类型：
--
作者：
T. Jenkins;R. Eisenthal;P. Weitzman
在布氏锥虫体内发现了两种琥珀酸硫代激酶活性，它们是腺嘌呤或鸟嘌呤核苷酸的特异体。关键的糖酵解和柠檬酸循环酶被测量，以显示糖酵解的抑制和柠檬酸循环相对于血流形式的原环状的去抑制。伴随着柠檬酸循环酶活性的增加，腺嘌呤连接的琥珀酸硫酸激酶活性显著增加。然而，鸟嘌呤连接的琥珀酸硫代激酶的活性仅略有增加。这些结果暗示腺嘌呤连接的酶是柠檬酸循环的重要组成部分，而鸟嘌呤连接的酶似乎受到单独的控制。这份函件还首次报告了布鲁氏毛滴虫血液(细长)形式中出现柠檬酸合成酶活性。
Two succinate thiokinase activities specific for either adenine or guanine nucleotides have been found in Trypanosoma brucei. Key glycolytic and citric acid cycle enzymes were measured to show repression of glycolysis and derepression of the citric acid cycle in the procyclic form, relative to the bloodstream form. A marked rise in adenine-linked succinate thiokinase activity accompanied a rise in activity of citric acid cycle enzymes. However, guanine-linked succinate thiokinase was found to increase only slightly in activity. These results implicate the adenine-linked enzyme as an essential component of the citric acid cycle, whereas the guanine-linked enzyme appears to be under separate control. This communication also reports for the first time the occurrence of citrate synthase activity in the bloodstream (long slender) form of T. brucei.