Preparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion.
Preparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion.
复制标题
DOI：
10.3389/fbioe.2021.759303
复制
发表时间：
2021
影响因子：
5.7
通讯作者：
Du X
中科院分区：
文献类型：
--
作者：
Zhao L;Wang H;Feng C;Song F;Du X
配戴隐形眼镜引起的微生物感染已成为一个主要的健康问题，因此抗菌隐形眼镜的设计和开发引起了广泛的关注。为了安全有效地抑制角膜接触镜的细菌粘附，我们采用原位自由基聚合法制备了表没食子儿茶素没食子酸酯（epigallocatechin gallate，EGCG）淀粉水凝胶/角膜接触透镜复合材料。紫外-可见分光光度法表征所得接触镜的足够透射率，并使用差示扫描量热法和热重分析法检查其令人满意的稳定性。然后进行细胞毒性实验和降解实验，研究角膜接触镜的生物相容性和降解性。结果表明，该复合材料无毒，可降解性好。此外，还对接触镜的体外释放EGCG的能力进行了评价，结果表明，这些接触镜中的EGCG可持续释放至少14天。进一步的细菌粘附试验表明，与对照相比，负载EGCG的淀粉水凝胶/角膜接触镜可以显著降低铜绿假单胞菌的粘附。EGCG负载的淀粉水凝胶/接触透镜复合物为预防眼部微生物感染和抑制细菌性角膜炎提供了潜在的干预策略。
Microbial infections caused by wearing contact lenses has become a major health problem, so the design and development of antibacterial contact lenses has attracted widespread attention. To safely and effectively inhibit bacterial adhesion of contact lenses, we have facilely prepared epigallocatechin gallate (EGCG) loaded starch hydrogel/contact lens composites by in-situ free radical polymerization of the mixture containing 2-hydroxylethyl methacrylate, methacrylic acid and ethylene glycol dimethacrylate. The adequate transmittance of the resulting contact lenses was characterized by ultraviolet-visible spectrophotometry, and their satisfactory stability was examined using differential scanning calorimetry and thermogravimetric analysis. Whereafter, cytotoxicity and degradation experiments were performed to investigate the biocompatibility and degradability of the contact lenses. The results showed the nontoxicity and good degradability of the composites. Besides, the capacity of the contact lenses for in vitro release of EGCG was also evaluated, and the results showed that the EGCG in these contact lenses can be sustainably released for at least 14 days. Further bacterial adhesion assay suggested that the EGCG loaded starch hydrogel/contact lenses could significantly reduce the adhesion of Pseudomonas aeruginosa compared to the control. The EGCG loaded starch hydrogel/contact lens composites provide a potential intervention strategy for preventing ocular microbial infections and inhibiting bacterial keratitis.