Evaluation of Genetic Variability in Algerian Clover (Trifolium L.) Based on Morphological and Isozyme markers
Evaluation of Genetic Variability in Algerian Clover (Trifolium L.) Based on Morphological and Isozyme markers
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基于形态学和同工酶标记的阿尔及利亚三叶草（Trifolium L.）遗传变异评价
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发表时间：
2016
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通讯作者：
D. Khelifi
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作者：
Imane Medoukali;I. Bellil;D. Khelifi
梅杜卡利岛贝利尔岛哈利菲湾（2015年）：阿尔及利亚三叶草（Trifolium L.）基于形态学和同工酶标记。Czech J. Genet.植物品种，51：50-61.利用形态学和同工酶标记分析了15个车轴草属植物种内和种间的遗传变异。大部分形态计量学特征对种的鉴别有贡献。采集地的环境与形态特征之间没有显著的关系。酯酶（EST）和谷草转氨酶（GOT）两种同工酶系统分析，证明多态性。同工酶标记的表型多样性在0.07 ~ 0.61之间，平均多态信息含量为0.31。两两的Jaccard的相似性系数范围在0.10和0.60之间，表明该集合代表遗传多样性物种。检测到了相当数量的种特异性酶谱，可用于菌种鉴定。同工酶标记的聚类模式与数量性状的聚类模式不一致。阿尔及利亚三叶草种间存在丰富的同工酶变异，可为育种提供良好的基因资源。
Medoukali I., Bellil I., Khelifi D. (2015): Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers. Czech J. Genet. Plant Breed., 51: 50–61. Genetic variation within and among fifteen Trifolium species represented by 157 accessions was assessed using morphological and isozyme markers. Most of morphometric characters contributed to the discrimination of the species. No significant relationship between the environment of the collection site and morphological features was detected. The two isozyme systems analysed, esterase (EST) and glutamate oxaloacetate transaminase (GOT), proved polymorphic. Phenotype diversity of isozyme markers ranged from 0.07 to 0.61 with an average of 0.31 based on the polymorphic information content. The pairwise Jaccard’s similarity coefficient ranged between 0.10 and 0.60, indicating that the collection represents genetically diverse species. A considerable number of species-specific zymograms were detected which can be used for the species identification. The clustering pattern of isozyme markers was incongruent with the groupings based on quantitative traits. The rich isozyme variability present among the Algerian clover species indicates that they can provide good gene resources for breeding.