Whole-Transcriptome Analysis of Yak and Cattle Heart Tissues Reveals Regulatory Pathways Associated With High-Altitude Adaptation.
Whole-Transcriptome Analysis of Yak and Cattle Heart Tissues Reveals Regulatory Pathways Associated With High-Altitude Adaptation.
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DOI：
10.3389/fgene.2021.579800
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发表时间：
2021
影响因子：
3.7
通讯作者：
Ji Q
中科院分区：
文献类型：
--
作者：
Wang H;Zhong J;Wang J;Chai Z;Zhang C;Xin J;Wang J;Cai X;Wu Z;Ji Q
然而，驱动这种低氧适应的调控网络仍然难以捉摸。选取分别位于高海拔地区（HAC）和低海拔地区（LAC）的两个本土牛种——雷洛奇牦牛（LWQY）及其亲缘牛（Bos Taurus）的心脏组织进行RNA测序。在LAC-vs-LWQY和LAC-vs-HAC对照组中，共发现178个共差异表达的蛋白编码转录本（co-DETs），包括NFATC2、NFATC1、ENPP2、ACSL4、BAD以及许多其他功能被报道与免疫系统、内分泌系统和脂质代谢相关的基因。在LAC-vs-LWQY和LAC-vs-HAC比较中，分别有2个和230个lncRNA转录本差异表达，但没有lncRNA转录本共差异表达。在共差异表达的58个mirna中，18个上调，40个下调。此外，在LAC-vs-LWQY和LAC-vs-HAC对照组中，分别有640个（501个上调，139个下调）和152个（152个上调，1个下调）circrna差异表达，共有53个共差异表达的circrna上调。多种co-DETs作为miRNAs/lncRNAs的靶点，在高海拔适应相关过程中显著富集，如T细胞受体信号、VEGF信号和cAMP信号。通过整合共差异表达mrna、miRNAs、lncRNAs和circRNAs之间的竞争关系，构建了竞争内源RNA （ceRNA）网络。此外，构建了与缺氧适应相关的ceRNA网络，并提出了6种mrna （MAPKAPK3、PXN、NFATC2、ATP7A、DIAPH1和F2R）、8种mirna（包括miR-195）和15种circrna（包括novel-circ-017096和novel-circ-018073）作为心脏缺氧适应调节的新候选物。综上所述，本研究记录的数据提供了对高原适应分子网络的新认识，以及参与这一生理过程的蛋白质编码转录物和非编码转录物的更详细信息，这些转录物在高原适应过程中相互“串扰”的详细机制值得未来的研究。
The yak (Bos grunniens) is an important livestock species that can survive the extremely cold, harsh, and oxygen-poor conditions of the Qinghai-Tibetan Plateau and provide meat, milk, and transportation for the Tibetans living there. However, the regulatory network that drive this hypoxic adaptation remain elusive. The heart tissues from LeiRoqi (LWQY) yak and their related cattle (Bos Taurus) breeds, which are two native cattle breeds located in high altitude (HAC) and low altitude (LAC) regions, respectively, were collected for RNA sequencing. A total of 178 co-differentially expressed protein-coding transcripts (co-DETs) were discovered in each of the LAC-vs-LWQY and LAC-vs-HAC comparison groups, including NFATC2, NFATC1, ENPP2, ACSL4, BAD, and many other genes whose functions were reported to be associated with the immune-system, endocrine-system, and lipid metabolism. Two and 230 lncRNA transcripts were differentially expressed in the LAC-vs-LWQY and LAC-vs-HAC comparisons’ respectively, but no lncRNA transcripts that were co-differentially expressed. Among the 58 miRNAs that were co-differentially expressed, 18 were up-regulated and 40 were down-regulated. In addition, 640 (501 up-regulated and 139 down-regulated) and 152 (152 up-regulated and one down-regulated) circRNAs showed differential expression in LAC-vs-LWQY and LAC-vs-HAC comparison groups, respectively, and 53 up-regulated co-differentially expressed circRNAs were shared. Multiple co-DETs, which are the targets of miRNAs/lncRNAs, are significantly enriched in high-altitude adaptation related processes, such as, T cell receptor signaling, VEGF signaling, and cAMP signaling. A competing endogenous RNA (ceRNA) network was constructed by integrating the competing relationships among co-differentially expressed mRNAs, miRNAs, lncRNAs and circRNAs. Furthermore, the hypoxic adaptation related ceRNA network was constructed, and the six mRNAs (MAPKAPK3, PXN, NFATC2, ATP7A, DIAPH1, and F2R), the eight miRNAs (including miR-195), and 15 circRNAs (including novel-circ-017096 and novel-circ-018073) are proposed as novel and promising candidates for regulation of hypoxic adaptation in the heart. In conclusion, the data recorded in the present study provides new insights into the molecular network of high-altitude adaptation along with more detailed information of protein-coding transcripts and non-coding transcripts involved in this physiological process, the detailed mechanisms behind how these transcripts “crosstalk” with each other during the plateau adaptation are worthy of future research efforts.
介质复合体对肌肉代谢的控制。
影响因子：
5.4
作者：
Amoasii L;Olson EN;Bassel-Duby R
通讯作者：
Bassel-Duby R