Tea flavanols inhibit angiotensin-converting enzyme activity and increase nitric oxide production in human endothelial cells
Tea flavanols inhibit angiotensin-converting enzyme activity and increase nitric oxide production in human endothelial cells
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DOI：
10.1211/jpp.58.8.0016
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发表时间：
2006-08-01
影响因子：
3.3
通讯作者：
Andersson, Rolf G. G.
中科院分区：
文献类型：
--
作者：
Persson, Ingrid A. L.;Josefsson, Martin;Andersson, Rolf G. G.
据报道，茶花对心血管系统具有多种药理作用：抗氧化、抗增殖和抗血管生成活性，以及一氧化氮合酶激活。本研究的目的是调查茶与血管紧张素转换酶 (ACE) 和一氧化氮 (NO) 之间的联系是否可以解释茶对心血管系统的药理作用。将培养的人脐静脉 (HUVEC) 内皮细胞分别与日本 Sencha（绿茶）、印度阿萨姆碎橙白毫（红茶）和 Rooibos 茶的提取物一起孵育。研究了绿茶和红茶中的主要黄烷醇和嘌呤生物碱对 ACE 和 NO 的影响。与绿茶、红茶和路易波士茶一起孵育 10 分钟后，绿茶和红茶对 HUVEC 中的 ACE 活性产生显着且剂量依赖性的抑制。 Rooibos 茶对 ACE 没有显着影响。与 (-)-表儿茶素、(-)-表没食子儿茶素、(-)-表儿茶素晚期和 (-)表没食子儿茶素没食子酸酯孵育 10 分钟后，所有四种茶儿茶素都观察到 HUVEC 中 ACE 活性的剂量依赖性抑制。培养 24 小时后，绿茶、红茶和路易波士茶对 HUVEC 中 NO 产生的剂量依赖性影响显着增加。与(-)-表儿茶素、(-)-表没食子儿茶素、(-)-表儿茶素没食子酸酯和(-)-表没食子儿茶素没食子酸酯孵育24小时后，观察到HUVEC中NO产量呈剂量依赖性增加。总之，茶树提取物可能具有预防和预防心血管疾病的潜力。
A diversity of pharmacological effects on the cardiovascular system have been reported for Camellia sinensis: antioxidative, antiproliferative and anti-angiogenic activity, and nitric oxide synthase activation. The purpose of this study was to investigate if the connection between tea and angiotensin converting enzyme (ACE) and nitric oxide (NO) might be an explanation of the pharmacological effects of tea on the cardiovascular system. Cultured endothelial cells from human umbilical veins (HUVEC) were incubated with extracts of Japanese Sencha (green tea), Indian Assam Broken Orange Pekoe (black tea) and Rooibos tea, respectively. The main flavanols and purine alkaloids in green and black tea were examined for their effects on ACE and NO. After incubation with green tea, black tea and Rooibos tea for 10 min, a significant and dose-dependent inhibition of ACE activity in HUVEC was seen with the green tea and the black tea. No significant effect on ACE was seen with the Rooibos tea. After 10-min incubation with (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechingal late and (-)epigallocatechingallate, a dose-dependent inhibition of ACE activity in HUVEC was seen for all four tea catechins. After 24-h incubation, a significantly increased dose-dependent effect on NO production in HUVEC was seen for the green tea, the black tea and the Rooibos tea. After 24-h incubation with (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechingallate and (-)-epigallocatechingallate, a dose-dependent increased NO production in HUVEC was seen. In conclusion, tea extracts from C. sinensis may have the potential to prevent and protect against cardiovascular disease.