Purification of sulforaphane from Brassica oleracea seed meal using low-pressure column chromatography
Purification of sulforaphane from Brassica oleracea seed meal using low-pressure column chromatography
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DOI：
10.1016/j.jchromb.2005.09.041
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发表时间：
2005-12-15
影响因子：
3
通讯作者：
Xiao, Q
中科院分区：
文献类型：
--
作者：
Liang, H;Yuan, QP;Xiao, Q
萝卜硫素是一种异硫氰酸酯，天然存在于广泛食用的甘蓝蔬菜中，并已被证明可以阻止肿瘤的形成。B.使用乙腈水溶液的线性梯度，通过RP-HPLC测定甘蓝种子（花椰菜、布鲁塞尔芽甘蓝、卷心菜、花椰菜和羽衣甘蓝）。一种低成本分离纯化B中天然莱菔硫烷的新方法。在这项工作中描述了甘蓝种子粉。首先从B中分离出萝卜硫烷粗品。采用乙酸乙酯、10%乙醇和正己烷不混溶溶剂萃取法从甘蓝籽粕中提取萝卜硫素，并以萝卜硫素粗品为原料，采用低压硅胶柱层析（200-300目），采用不同洗脱剂和洗脱方式制备高纯度萝卜硫素。通过对不同洗脱方法的比较，认为梯度洗脱法优于等度洗脱法，可缩短洗脱时间，减少洗脱剂用量，提高莱菔硫烷产品纯度。采用梯度洗脱法，莱菔硫烷的纯度和回收率均大于90%。(c)2005 Elsevier B. V.保留所有权利。
Sulforaphane is an isothiocyanate that is present naturally in widely consumed Brassica oleracea vegetables and has been shown to block the formation of tumors. The contents of sulforaphane in five groups of B. oleracea seeds (broccoli, Brussels sprouts, cabbage, cauliflower and kale) were determined by RP-HPLC using linear gradient of acetonitrile in water. A new low-cost method to isolate and purify natural sulforaphane from B. oleracea seed meal was described in this work. Crude sulforaphane was first separated from B. oleracea seed meal by using immiscible solvent extraction with ethyl acetate, 10% ethanol and hexane, and the crude sulforaphane was used as raw materials to prepare high purity sulforaphane by low-pressure column chromatography of silica gel (200-300 mesh) with different eluents and elution modes. Compared with these different elution methods, the gradient elution was preferable to the isocratic elution for reducing the elution time and the eluent consumption and increasing the purity of sulforaphane product. The purity and recovery of sulforaphane were more than 90% in gradient elution. (c) 2005 Elsevier B.V. All rights reserved.