Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata)
Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata)
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DOI：
10.1007/s11032-011-9656-9
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发表时间：
2012-08
影响因子：
3.1
通讯作者：
QingBiao Wang;Yang-yong Zhang;Z. Fang;Yumei Liu;Limei Yang;M. Zhuang
中科院分区：
文献类型：
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作者：
QingBiao Wang;Yang-yong Zhang;Z. Fang;Yumei Liu;Limei Yang;M. Zhuang
6种不同类型甘蓝异体细胞质雄性不育（CMS）的单序列重复序列（SSR）分析。利用来自拟南芥叶绿体（cp）基因组的32对SSR引物和来自拟南芥叶绿体(b)基因组的21对SSR引物，得到了变种（var.capitata）。Napusmitochondrial （mt）基因组序列。共有11条cpSSR和4条mtSSR引物在NigCMS、OguCMSR1、OguCMSR2、OguCMSR3、OguCMSHY和PolCMS 6个白菜CMS类型中发现多态性。聚类分析表明，仅用3组引物ACP43、ACP47、mtSSR2就能明确区分6个白菜CMS类型。经分析，所选扩增子序列与inA序列具有较高的一致性。芥,B。rapaandB。显著。对准聚类分析显示，多态性主要包括SSR数量变异、单核苷酸多态性（SNP）和序列插入或缺失（InDel）。结果表明，特异的线粒体或叶绿体SSR分析是白菜CMS类型鉴定的可行替代方法。
The profiles of single sequence repeat (SSR) in six distinct allo-cytoplasmic male sterile (CMS) types of cabbage (Brassica oleraceaL. var.capitata) were generated using 32 SSR primer pairs derived from theArabidopsis thalianachloroplast (cp) genome and another 21 SSR primers from theB. napusmitochondrial (mt) genome sequences. In total, 11 cpSSR and 4 mtSSR primers revealed polymorphism among the six cabbage CMS types, namely NigCMS, OguCMSR1, OguCMSR2, OguCMSR3, OguCMSHY and PolCMS. Through cluster analysis, six cabbage CMS types could be unambiguously differentiated with just three sets of primers (ACP43, ACP47, mtSSR2). Analysis of the selected amplicon sequences showed high identity to that of the corresponding sequences inA. thaliana,B. rapaandB. napus. The aligned cluster analysis revealed that the polymorphism mainly included SSR number variation, single nucleotide polymorphism (SNP), and sequence insertion or deletion (InDel). Our results demonstrated that specific mitochondrial or chloroplast SSR analysis could be a feasible alternative means for cabbage CMS type identification.