Specific changes in the pattern of protein synthesis during meiotic maturation of mammalian oocytes in vitro.
Specific changes in the pattern of protein synthesis during meiotic maturation of mammalian oocytes in vitro.
复制标题
哺乳动物卵母细胞体外减数分裂成熟过程中蛋白质合成模式的具体变化。
DOI：
10.1073/pnas.74.2.538
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发表时间：
1977
影响因子：
11.1
通讯作者：
P. M. Wassarman
中科院分区：
文献类型：
--
作者：
R. Schultz;P. M. Wassarman
利用高分辨率双向电泳技术研究了小鼠卵母细胞体外成熟过程中蛋白质合成的模式。[35 S]蛋氨酸标记的卵母细胞蛋白的荧光图显示，减数分裂进程从网状细胞中期II（减数分裂成熟）是伴随着显着的变化，由卵母细胞合成的蛋白质的模式。几乎所有观察到的变化都发生在卵母细胞萌发囊泡破裂之后，但并不依赖于其他形态学事件的发生，如纺锤体形成或极体发射。这些蛋白质合成的变化不发生在不能自发地经历胚泡破裂的卵母细胞中或在被二丁酰3 '：5'-环AMP阻滞在胚泡阶段的卵母细胞中。这些数据表明，卵母细胞的核质和细胞质的混合可能会引发许多蛋白质合成的变化，伴随小鼠卵母细胞体外减数分裂成熟。
High resolution two-dimensional electrophoresis has been used to examine the pattern of protein synthesis during meiotic maturation of mouse oocytes in vitro. Fluorograms of [35S]methionine-labeled oocyte proteins have revealed that meiotic progression from dictyate to metaphase II (meiotic maturation) is accompanied by marked changes in the pattern of proteins synthesized by oocytes. Virtually all of the changes observed take place subsequent to the breakdown of the oocyte's germinal vesicle, but are not dependent upon the occurrence of other morphological events, such as spindle formation or polar body emission. These changes in protein synthesis do not take place in oocytes that fail to undergo breakdown of germinal vesicles spontaneously or in oocytes arrested at the germinal vesicle stage by dibutyryl 3':5'-cyclic AMP. These data suggest that mixing of the oocyte's nucleoplasm and cytoplasm may trigger many of the changes in protein synthesis that accompany meiotic maturation of mouse oocytes in vitro.