Cellular stiffness response to external deformation: Tensional homeostasis in a single fibroblast
Cellular stiffness response to external deformation: Tensional homeostasis in a single fibroblast
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DOI：
10.1002/cm.20037
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发表时间：
2004-12-01
影响因子：
--
通讯作者：
Kawabata, K
中科院分区：
文献类型：
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作者：
Mizutani, T;Haga, H;Kawabata, K
通过扫描探针显微镜测量成纤维细胞的刚度响应，随后通过弹性基底变形8%进行伸长或压缩。细胞刚度，反映细胞内的张力作用沿着应力纤维，立即减少或增加响应于拉伸或压缩刺激，分别。在这种快速变化之后，成纤维细胞在接下来的2小时内逐渐恢复到其初始硬度，然后稳定。在SPM测量过程中，细胞在8%变形后没有显示出明显的形状变化。GFP-肌动蛋白的荧光检测表明，应力纤维的结构没有被这种小程度的变形明显改变。用Y-27632治疗以抑制肌球蛋白磷酸化并消除细胞收缩性，消除了机械伸长后硬度的变化。这些结果表明，成纤维细胞具有调节细胞内张力的机制，沿着应力纤维，以维持细胞刚度在一个恒定的平衡状态。(C)2004 Wiley-Liss，Inc.
Stiffness responses of fibroblasts were measured by scanning probe microscopy, following elongation or compression by deformation of an elastic substrate by 8%. The cellular stiffness, reflecting intracellular tension acting along stress fibers, decreased or increased instantly in response to the elongating or compressing stimuli, respectively. After this rapid change, the fibroblasts gradually recovered to their initial stiffness during the following 2 h, and then stabilized. The cells did not show conspicuous changes in shape after the 8% deformation during the SPM measurements. Fluorescence examination for GFP-actin demonstrated that the structure of the stress fibers was not altered noticeably by this small degree of deformation. Treatment with Y-27632, to inhibit myosin phosphorylation and abrogate cellular contractility, eliminated the change in stiffness after the mechanical elongation. These results indicate that fibroblasts possess a mechanism that regulates intracellular tension along stress fibers to maintain the cellular stiffness in a constant equilibrium state. (C) 2004 Wiley-Liss, Inc.