CLONING OF THE AMILORIDE-SENSITIVE FMRFAMIDE PEPTIDE-GATED SODIUM-CHANNEL
CLONING OF THE AMILORIDE-SENSITIVE FMRFAMIDE PEPTIDE-GATED SODIUM-CHANNEL
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DOI：
10.1038/378730a0
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发表时间：
1995-12-14
期刊：
影响因子：
64.8
通讯作者：
BARBRY, P
中科院分区：
文献类型：
--
作者：
LINGUEGLIA, E;CHAMPIGNY, G;BARBRY, P
肽Phe-Met-Arg-Phe-NH2 （FMRFamide）和结构相关肽存在于无脊椎动物和脊椎动物的神经系统中（1,2）。虽然它们构成了无脊椎动物肽神经递质的主要类别(3)，但其受体的分子结构尚未被确定。在蜗牛螺旋(4)的神经元中，以及在海雀(5)和运动(6)神经元中，FMRFamide由于直接激活酰胺敏感的Na+通道而诱导快速兴奋性去极化反应(4)。我们现在已经从螺旋神经组织中分离出一个互补的DNA；当在爪蟾卵母细胞中表达时，它编码一个fmrf -酰胺激活的Na+通道（FaNaCh），该通道可以被阿米洛利阻断。相应的蛋白与先前克隆的上皮Na+通道亚基（7-12）和秀丽隐杆线虫（13-15）具有非常低的序列同源性，但其整体结构组织相同。据我们所知，这是第一次对肽门控的嗜离子受体进行表征。
THE peptide Phe-Met-Arg-Phe-NH2 (FMRFamide) and structurally related peptides are present both in invertebrate and vertebrate nervous systems(1,2). Although they constitute a major class of invertebrate peptide neurotransmitters(3), the molecular structure of their receptors has not yet been identified, In neurons of the snail Helix aspersa(4), as well as in Aplysia bursting(5) and motor(6) neurons, FMRFamide induces a fast excitatory depolarizing response due to direct activation of an amiloride-sensitive Na+ channel(4). We have now isolated a complementary DNA from Helix nervous tissue; when expressed in Xenopus oocytes, it encodes an FMRF-amide-activated Na+ channel (FaNaCh) that can be blocked by amiloride. The corresponding protein shares a very low sequence identity with the previously cloned epithelial Na+ channel subunits(7-12) and Caenorhabditis elegans degenerins(13-15), but it displays the same overall structural organization. To our knowledge, this is the first characterization of a peptide-gated ionotropic receptor.