The Tissue Distribution of Nesfatin-1/NUCB2 in Mouse.
The Tissue Distribution of Nesfatin-1/NUCB2 in Mouse.
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DOI：
10.12717/devrep.2014.18.4.301
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Yang H
中科院分区：
文献类型：
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作者：
Kim J;Chung Y;Kim H;Im E;Lee H;Yang H
Nesfatin-1 是一种厌食核结合素 2 (NUCB2) 衍生的下丘脑肽，控制食欲和能量代谢。最近的研究表明nesfatin-1/NUCB2不仅在大脑中表达，还在胃和脂肪组织中表达。因此，我们通过实时PCR、蛋白质印迹和免疫组织化学染色研究了nesfatin-1/NUCB2在雄性和雌性小鼠的各个组织中的分布。实时PCR分析显示，NUCB2 mRNA主要在雄性和雌性小鼠的垂体中表达，在下丘脑、脾脏、胸腺、心脏、肝脏和肌肉中表达水平较低。睾丸、附睾、卵巢和子宫等生殖器官中的表达量远高于下丘脑。在雄性和雌性小鼠中，nesfatin-1 蛋白水平的蛋白质印迹分析结果与实时 PCR 分析结果相似。这些结果表明nesfatin-1/NUCB2在内分泌和非内分泌器官中具有广泛的生理作用。此外，免疫组织化学染色显示 nesfatin-1 定位于间质细胞，包括 Leydig 细胞和附睾柱状上皮。 Nesfatin-1也在卵巢的卵泡膜细胞和间质细胞以及子宫内膜和子宫腺体的上皮细胞中表达。这些结果表明，nesfatin-1 是男性和女性生殖器官中类固醇生成和性腺功能的新型有效调节剂。需要进一步的研究来阐明nesfatin-1在雄性和雌性小鼠的各个器官中的功能。
Nesfatin-1, an anorexic nucleobindin-2 (NUCB2)-derived hypothalamic peptide, controls appetite and energy metabolism. Recent studies show that nesfatin-1/NUCB2 is expressed not only in the brain but also in gastric and adipose tissues. Thus, we investigated the distributions of nesfatin-1/NUCB2 in various tissues of male and female mice by real-time PCR, western blotting, and immunohistochemical staining. Real-time PCR analyses showed that NUCB2 mRNA was predominantly expressed in the pituitary and at lower levels in the hypothalamus, spleen, thymus, heart, liver, and muscle of both male and female mice. Expression was much higher in reproductive organs, such as the testis, epididymis, ovary, and uterus, than in the hypothalamus. Western blot analysis of the nesfatin-1 protein level showed similar results to the real-time PCR analyses in both male and female mice. These results suggest that nesfatin-1/NUCB2 have widespread physiological effects in endocrine and non-endocrine organs. In addition, immunohistochemical staining revealed that nesfatin-1 was localized in interstitial cells, including Leydig cells and in the columnar epithelium of the epididymis. Nesfatin-1 was also expressed in theca cells and interstitial cells in the ovary and in epithelial cells of the endometrium and uterine glands in the uterus. These results suggest that nesfatin-1 is a novel potent regulator of steroidogenesis and gonadal function in male and female reproductive organs. Further studies are required to elucidate the functions of nesfatin-1 in various organs of male and female mice.