Effects of Long-Term Dietary Soy Treatment on Female Urethral Morphology and Function in Ovariectomized Nonhuman Primates
Effects of Long-Term Dietary Soy Treatment on Female Urethral Morphology and Function in Ovariectomized Nonhuman Primates
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DOI：
10.1016/j.juro.2008.07.021
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发表时间：
2008-11-01
影响因子：
6.6
通讯作者：
Badlani, Gopal
中科院分区：
文献类型：
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作者：
Gratzke, Christian;Jarajapu, Yagna P. R.;Badlani, Gopal
目的：雌激素对女性尿道的激动作用包括增加收缩功能、血流量和粘膜增生。这种效果是否可以通过大豆为基础的植物雌激素饮食实现尚不清楚。我们研究了慢性植物雌激素治疗对卵巢切除猴尿道的结构和功能特性的影响。材料和方法：卵巢切除后，18只猴子被喂食含有大豆（9）或酪蛋白（9）为基础的蛋白质的饮食32个月。尸检时，取出尿道和膀胱，并将尿道分成3个等长段，包括近端、中间和远端段。每个尿道段和1膀胱段进行了测试，在体外的功能反应，电场刺激和药物刺激，和近端到远端段进行了测试，尿路上皮厚度和粘膜area.Results：电场刺激产生频率依赖性收缩反应，在膀胱，近端和中间段，但不是在远端段。卡巴胆碱，苯肾上腺素和内皮素-1产生浓度依赖性收缩的所有尿道段。最大反应从近端到远端逐渐降低（p
Purpose: Agonistic effects of estrogen on the female urethra include an increase in contractile function, blood flow and mucosal hyperplasia. Whether such effects can be achieved by soy based phytoestrogen diets is unclear. We studied the effects of chronic phytoestrogen treatment on the structural and functional properties of the urethra in ovariectomized monkeys.Materials and Methods: Following ovariectomy 18 monkeys were fed a diet containing soy (9) or casein (9) based protein for 32 months. At necropsy the urethra and bladder were removed and the urethra was separated into 3 segments of equal length, including a proximal, a middle and a distal segment. Each urethral segment and 1 bladder segment was tested in vitro for functional responses to electrical field stimulation and pharmacological stimulation, and the proximal to distal segments were tested for urothelial thickness and mucosal area.Results: Electrical field stimulation produced frequency dependent contractile responses in the bladder, proximal and middle segments but not in the distal segment. Carbachol, phenylephrine and endothelin-1 produced concentration dependent contractions in all urethral segments. The maximum response decreased from the proximal to the distal segment (p