MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines.
MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines.
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DOI：
10.1194/jlr.s087163
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发表时间：
2018-10
影响因子：
6.5
通讯作者：
Wenk MR
中科院分区：
文献类型：
--
作者：
Burla B;Arita M;Arita M;Bendt AK;Cazenave-Gassiot A;Dennis EA;Ekroos K;Han X;Ikeda K;Liebisch G;Lin MK;Loh TP;Meikle PJ;Orešič M;Quehenberger O;Shevchenko A;Torta F;Wakelam MJO;Wheelock CE;Wenk MR
人类血液是一种自我再生的富含脂质的生物液体，通常在医院环境中收集。血浆中发现的脂质分子（血浆脂质组）的库存提供了对健康和疾病中个体代谢和生理学的见解。血浆脂质组的紊乱也发生在与脂质代谢没有直接联系的情况下;因此，基于MS的血浆脂质组学是一系列临床诊断和疾病管理中的新兴工具。然而，在将此类脂质组学数据转化为临床应用方面存在挑战。这些涉及脂质定量的重现性、准确度和精密度、研究设计、样品处理和数据共享。这份立场文件来自一个研讨会，该研讨会发起了一个社区主导的过程，以制定和定义一套普遍接受的血浆或血清定量MS脂质组学指南，并将统一独立实验室不同仪器平台上获得的数据作为最终目标。我们希望其他领域也能受益于并遵循这一先例。
Human blood is a self-regenerating lipid-rich biological fluid that is routinely collected in hospital settings. The inventory of lipid molecules found in blood plasma (plasma lipidome) offers insights into individual metabolism and physiology in health and disease. Disturbances in the plasma lipidome also occur in conditions that are not directly linked to lipid metabolism; therefore, plasma lipidomics based on MS is an emerging tool in an array of clinical diagnostics and disease management. However, challenges exist in the translation of such lipidomic data to clinical applications. These relate to the reproducibility, accuracy, and precision of lipid quantitation, study design, sample handling, and data sharing. This position paper emerged from a workshop that initiated a community-led process to elaborate and define a set of generally accepted guidelines for quantitative MS-based lipidomics of blood plasma or serum, with harmonization of data acquired on different instrumentation platforms across independent laboratories as an ultimate goal. We hope that other fields may benefit from and follow such a precedent.