STRUCTURE OF LAMELLAR LIPID DOMAINS AND CORNEOCYTE ENVELOPES OF MURINE STRATUM-CORNEUM - AN X-RAY-DIFFRACTION STUDY
STRUCTURE OF LAMELLAR LIPID DOMAINS AND CORNEOCYTE ENVELOPES OF MURINE STRATUM-CORNEUM - AN X-RAY-DIFFRACTION STUDY
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DOI：
10.1021/bi00410a031
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发表时间：
1988-05-17
期刊：
影响因子：
2.9
通讯作者：
KING, GI
中科院分区：
文献类型：
--
作者：
WHITE, SH;MIREJOVSKY, D;KING, GI
皮肤最外层的脂质大部分局限于角质层角质细胞周围的细胞外空间，在那里它形成多层粘附基质以充当皮肤的主要渗透屏障。这些细胞间结构域的分子结构的知识对于理解各种皮肤病理及其治疗、经皮药物递送和皮肤的美容维护是重要的。我们已经通过X射线衍射研究了在25、45和70 ° C下鼠角质层（SC）的细胞间结构域和提取的脂质的结构。C，其为已知热相变附近的温度[Alberfeld，S. J.，和Elias，P. M.（1982）J. Invest. Dermatol. 79，1-3]。细胞间区域产生具有131 ± 1的布拉格间距的层状衍射图案。2. ANG..然而，从SC中提取并分散在过量水中的脂质在研究的任何温度下都不会产生简单的层状衍射图案。这一事实和其他事实表明，另一种成分，可能是一种蛋白质，必须控制结构的细胞间脂质结构域。我们还获得了衍射图案归因于蛋白包膜的角质细胞。这些图案表明是β-折叠的片材组织方案。没有观察到可归因于角蛋白的衍射图案。
The lipid of the outermost layer of the skin is confined largely to the extracellular spaces surrounding the corneocytes of the stratum corneum where it forms a multilamellar adhesive matrix to act as the major permeability barrier of the skin. Knowledge of the molecular architecture of these intercellular domains is important for understanding various skin pathologies and their treatment, percutaneous drug delivery, and the cosmetic maintenance of the skin. We have surveyed by X-ray diffraction the structure of the intercellular domains and the extracted lipids of murine stratum corneum (SC) at 25, 45 and 70.degree. C which are temperatures in the vicinity of known thermal phase transitions [Rehfeld, S. J., and Elias, P. M. (1982) J. Invest. Dermatol. 79, 1-3]. The intercellular domains produce lamellar diffraction patterns with a Bragg spacing of 131 .+-. 2 .ANG.. Lipid extracted from the SC and dispersed in excess water does not produce a simple lamellar diffraction pattern at any temperature studied, however. This and other facts suggest that another component, probably a protein, must be present to control the architecture of the intercellular lipid domains. We have also obtained diffraction patterns attributable to the protein envelopes of the corneocytes. The patterns suggest a .beta.-pleated sheet organizational scheme. No diffraction patterns were observed that could be attributed to keratin.