High soil pH enhances the network interactions among bacterial and archaeal microbiota in alpine grasslands of the Tibetan Plateau
High soil pH enhances the network interactions among bacterial and archaeal microbiota in alpine grasslands of the Tibetan Plateau
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DOI：
10.1111/1462-2920.15333
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发表时间：
2020-11-28
影响因子：
5.1
通讯作者：
Ma, Xiaojun
中科院分区：
文献类型：
--
作者：
Chen, Beibei;Jiao, Shuo;Ma, Xiaojun
土壤功能和过程是由复杂的微生物相互作用驱动的。因此，了解土壤微生物群的共存模式至关重要，特别是在脆弱的高山生态系统中。基于高通量测序技术，对西藏高寒草原土壤微生物共生网络的网络级拓扑特征进行了分析。我们验证了土壤pH值是预测土壤微生物共生网络网络级拓扑特征的最重要环境变量。土壤微生物群之间的关联性随着pH值的增加而增强（5.17-8.92），并且网络在中性pH下最稳定。此外，节点级拓扑特征表明，与细菌操作分类单元相比，古细菌操作分类单元在共生网络中起着核心作用。网络层次的拓扑特征揭示了草原生态系统中土壤微生物群之间的联系比草甸生态系统中的联系更紧密。因此，我们的研究表明，土壤pH值作为一个关键的环境过滤器，影响潜在的协会和生态特征的土壤微生物群在西藏高寒草原。这些发现提供了一个新的视角，独特的共生网络的地理模式，探索土壤微生物群的生态作用，从而帮助管理土壤细菌和古菌群落提供高山生态系统服务。
Soil functions and processes are driven by complex microbial interactions. It is, therefore, critical to understand the coexistence patterns of soil microbiota, especially in fragile alpine ecosystems. We identified biogeographic patterns in the network-level topological features of the soil microbial co-occurrence network in the Tibetan alpine grasslands, based on high-throughput sequencing. We verified that soil pH was the most important environmental variable for predicting network-level topological features of soil microbial co-occurrence networks. Associations among soil microbiota were enhanced with increasing pH (5.17-8.92), and the network was the most stable at neutral pH. Moreover, node-level topological features suggested that the archaeal operational taxonomic units, compared with bacterial operational taxonomic units, hold a central role in the co-occurrence network. Network-level topological features revealed closer connections among soil microbiota in the steppe ecosystem than in the meadow ecosystem. Therefore, our study demonstrated that soil pH served as a critical environmental filter that influenced the potential associations and ecological signature of soil microbiota in the Tibetan alpine grasslands. These findings provide a new perspective on the distinct biogeographic patterns of co-occurrence networks, to explore the ecological role of soil microbiota and thus help manage soil bacterial and archaeal communities for provisioning alpine ecosystem services.