Effects of Developmental Exposure to TiO2 Nanoparticles on Synaptic Plasticity in Hippocampal Dentate Gyrus Area: an In Vivo Study in Anesthetized Rats
Effects of Developmental Exposure to TiO2 Nanoparticles on Synaptic Plasticity in Hippocampal Dentate Gyrus Area: an In Vivo Study in Anesthetized Rats
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发育期暴露于 TiO2 纳米颗粒对海马齿状回区域突触可塑性的影响：麻醉大鼠体内研究
DOI：
10.1007/s12011-011-8990-4
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发表时间：
2011-12-01
影响因子：
3.9
通讯作者：
Wang, Ming
中科院分区：
文献类型：
--
作者：
Gao, Xiaoyan;Yin, Shuting;Wang, Ming
随着纳米二氧化钛(TiO2NPs)在工业和日常生活中的应用越来越广泛，越来越多的研究表明，纳米TiO2NPs可能对生物体的呼吸或代谢循环系统产生负面影响，而对大脑中枢神经系统(CNS)的研究却很少。海马区突触的可塑性被认为与中枢神经系统的某些高功能有关，如学习和记忆。因此，在本研究中，我们利用在体电生理记录，研究了发育过程中暴露于纳米二氧化钛对大鼠海马齿状回(DG)区突触可塑性的影响。测量输入/输出(I/O)功能、双脉冲反应(PPR)、场兴奋性突触后电位和群体峰电位。结果表明，与对照组相比，哺乳期纳米二氧化钛染毒仔鼠的I/O功能、PPR和长时程增强均减弱。然而，在妊娠期纳米二氧化钛暴露组中，只有PPR显著减弱。这些结果表明，发育过程中暴露于纳米二氧化钛可能会影响子代在体海马DG区的突触可塑性，这表明发育中的大脑，特别是哺乳期的大脑，对纳米二氧化钛暴露是敏感的。本研究揭示了纳米二氧化钛在中枢神经系统中的潜在毒性。为纳米二氧化钛的安全生产和应用提供参考，并为今后的毒理学研究提供参考。
With the increasing applications of titanium dioxide nanoparticles (TiO2 NPs) in industry and daily life, an increasing number of studies showed that TiO2 NPs may have negative effects on the respiratory or metabolic circle systems of organisms, while very few studies focused on the brain central nervous system (CNS). Synaptic plasticity in hippocampus is believed to be associated with certain high functions of CNS, such as learning and memory. Thus, in this study, we investigated the effects of developmental exposure to TiO2 NPs on synaptic plasticity in rats' hippocampal dentate gyrus (DG) area using in vivo electrophysiological recordings. The input/output (I/O) functions, paired-pulse reaction (PPR), field excitatory postsynaptic potential, and population spike amplitude were measured. The results showed that the I/O functions, PPR, and long-term potentiation were all attenuated in lactation TiO2 NPs-exposed offspring rats compared with those in the control group. However, in the pregnancy TiO2 NPs exposure group, only PPR was attenuated significantly. These findings suggest that developmental exposure to TiO2 NPs could affect synaptic plasticity in offspring's hippocampal DG area in vivo, which indicates that developmental brains, especially in lactation, are susceptible to TiO2 NPs exposure. This study reveals the potential toxicity of TiO2 NPs in CNS. It may give some hints on the security of TiO2 NPs production and application and shed light on its future toxicological studies.