Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in cold-tolerant genotypes of rice (Oryza sativa L.)
Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in cold-tolerant genotypes of rice (Oryza sativa L.)
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DOI：
10.1007/s00122-009-1226-8
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发表时间：
2010-03-01
影响因子：
5.4
通讯作者：
Jena, K. K.
中科院分区：
文献类型：
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作者：
Suh, J. P.;Jeung, J. U.;Jena, K. K.
低温或冷胁迫是温带水稻种植国家和热带高海拔地区水稻生产和生产力的主要限制因素之一。尽管低温影响水稻生长的各个阶段，但结实率受到严重损害，这显著降低了品种的产量潜力。本研究以耐冷新品系IR 66160 -121-4-4-2为供体，与冷敏感品种京苗杂交，获得153个F-8重组自交系，并对其进行数量性状基因座（QTL）分析。利用175个多态性SSR标记和复合区间作图进行QTL分析，在第3、7和9染色体上发现了3个主效QTL（qPSST-3、qPSST-7和qPSST-9）。SSR标记RM 569、RM 1377和RM 24545与所鉴定的关于在田间使用冷水（18- 19 A ° C）灌溉和在温室中控制空气温度（17 A ° C）的结实率的耐冷性QTL连锁。3个QTL对耐冷性表型变异的贡献率为27.4%。利用连锁DNA标记和单倍型分析验证了低温胁迫下结实率高的RILs，揭示了热带粳稻品种Jimbrug（Javanica）和温带粳稻品种沈农89 -366的祖先基因组的贡献。鉴定出3个由耐冷亲本贡献的QTL，它们对冷处理下的结实率表现出加性效应。本研究证明了一种新的表型鉴定方法以及与QTL相关的SSR标记的实用性，以选择耐冷基因型，以提高温带水稻产量。
Low temperature or cold stress is one of the major constraints of rice production and productivity in temperate rice-growing countries and high-altitude areas in the tropics. Even though low temperature affects the rice plant in all stages of growth, the percent seed set is damaged severely by cold and this reduces the yield potential of cultivars significantly. In this study, a new source of cold-tolerant line, IR66160-121-4-4-2, was used as a donor parent with a cold-sensitive cultivar, Geumobyeo, to produce 153 F-8 recombinant inbred lines (RILs) for quantitative trait locus (QTL) analysis. QTL analysis with 175 polymorphic simple sequence repeat (SSR) markers and composite interval mapping identified three main-effect QTLs (qPSST-3, qPSST-7, and qPSST-9) on chromosomes 3, 7, and 9. The SSR markers RM569, RM1377, and RM24545 were linked to the identified QTLs for cold tolerance with respect to percent seed set using cold-water (18-19A degrees C) irrigation in the field and controlled air temperature (17A degrees C) in the greenhouse. The total phenotypic variation for cold tolerance contributed by the three QTLs was 27.4%. RILs with high percent seed set under cold stress were validated with linked DNA markers and by haplotype analysis that revealed the contribution of progenitor genomes from the tropical japonica cultivar Jimbrug (Javanica) and temperate japonica cultivar Shen-Nung89-366. Three QTLs contributed by the cold-tolerant parent were identified which showed additive effect on percent seed set under cold treatment. This study demonstrated the utility of a new phenotyping method as well as the identification of SSR markers associated with QTLs for selection of cold-tolerant genotypes to improve temperate rice production.